Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3056Y] to Cathepsin D - BSA and Azide free
- Suitable for: Flow Cyt, ICC, IHC-P, WB
- Knockout validated
- Reacts with: Human
Product nameAnti-Cathepsin D antibody [EPR3056Y] - BSA and Azide free
See all Cathepsin D primary antibodies
DescriptionRabbit monoclonal [EPR3056Y] to Cathepsin D - BSA and Azide free
Tested applicationsSuitable for: Flow Cyt, ICC, IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Cathepsin D aa 50-150 (N terminal).
- WB: A431, HepG2 and MCF7 whole cell lysate
ab207550 is the carrier-free version of ab75811 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab207550 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab207550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).|
FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Tissue specificityExpressed in the aorta extrcellular space (at protein level).
Involvement in diseaseCeroid lipofuscinosis, neuronal, 10
Sequence similaritiesBelongs to the peptidase A1 family.
Contains 1 peptidase A1 domain.
modificationsN- and O-glycosylated.
Cellular localizationLysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
- Information by UniProt
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
All lanes : Anti-Cathepsin D antibody [EPR3056Y] (ab75811) at 1/1000 dilution
Lane 1 : Wild-type A431 whole cell lysate
Lane 2 : CTSD knockout A431 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lane 4 : MCF7 whole cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 46 kDa
Observed band size: 44 kDa why is the actual band size different from the predicted?
ab75811 was shown to specifically react with in wild-type A431 cells as signal was lost in CTSD knockout cells. Wild-type and CTSD knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab75811 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75811).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab207550 has not yet been referenced specifically in any publications.