Overview

  • Product name

    Cathepsin E Activity Assay Kit (Fluorometric)
    See all Cathepsin E kits
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate, Purified protein, Tissue Lysate
  • Assay type

    Enzyme activity (quantitative)
  • Sensitivity

    1000 µU
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    Cathepsin E Activity Assay Kit (ab211081) provides a convenient method for detecting Cathepsin E activity in tissue and cell lysates. It is based on the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorimeter or fluorescence microplate reader at Ex/Em = 320/420 nm.


    This assay kit is simple, rapid and can detect as low as 1 mU of Cathepsin E activity in biological samples.

  • Notes

    Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    CTSE Assay Buffer 1 x 25ml
    CTSE Lysis Buffer 1 x 25ml
    CTSE Substrate 1 x 200µl
    Human Cathepsin E (Positive Control) (50 µg) 1 vial
    MCA Standard (1 mM) 1 x 25µl
  • Research areas

  • Function

    May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.
  • Tissue specificity

    Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.
  • Sequence similarities

    Belongs to the peptidase A1 family.
  • Post-translational
    modifications

    Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide.
    Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.
  • Cellular localization

    Endosome. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.
  • Information by UniProt
  • Alternative names

    • CATE
    • CATE_HUMAN
    • Cathepsin E
    • Cathepsin E form II
    • CTSE
    • Erythrocyte membrane aspartic proteinase
    • Slow moving proteinase
    see all

Images

  • Typical MCA standard calibration curve.

  • Cathepsin E activity in rat liver lysate (6 µg), rat kidney lysate (12 µg) and positive control (2 µL). Background control (blank standard); sample background (sample control without reaction mix).

Protocols

References

ab211081 has not yet been referenced specifically in any publications.

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