A synthetic peptide made to the C-terminus of mouse Cathepsin E.
Murine reticulocyte lysate. Cathepsin E is expressed abundantly in the stomach, Clara cells and alveolar macrophages of the lung, brain microglia, spleen, and activated B lymphocytes. It is not expressed in resting B lymphocytes.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 42 kDa.
Use at an assay dependent concentration.
May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.
Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.
Belongs to the peptidase A1 family.
Glycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide. Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.
Endosome. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.
Western blot - Anti-Cathepsin E antibody (ab36996)
Anti-Cathepsin E antibody (ab36996) at 1/1000 dilution + Murine reticulocyte lysate at 10 µg
Predicted band size: 42 kDa Observed band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin E antibody (ab36996)This image is courtesy of an Abreview submitted by Rudolf Jung
ab36996 staining Cathepsin E at a dilution of 1/2000 in Human stomach and tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). The sections were PFA-fixed and subjected to Heat-mediated antigen retrieval in TRIS EDTA Buffer pH 9.0 and permeabilization with DAKO wash buffer with Tween. The primary antibody was diluted 1/2000 in DAKO blocking dilution buffer and incubated with the sample for 30 minutes at 20°C. An HRP-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody.
Fisher OM et al. High Expression of Cathepsin E in Tissues but Not Blood of Patients with Barrett's Esophagus and Adenocarcinoma. Ann Surg Oncol22:2431-8 (2015).
Read more (PubMed: 25348778) »
Fristrup N et al. Cathepsin E, maspin, Plk1, and survivin are promising prognostic protein markers for progression in non-muscle invasive bladder cancer. Am J Pathol180:1824-34 (2012).
Read more (PubMed: 22449953) »