Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Product nameCathepsin E Inhibitor Assay Kit (Fluorometric)
See all Cathepsin E kits
Sample typeInhibitor compounds
Cathepsin E Inhibitor Screening Kit (Fluorometric) (ab211082) provides a rapid, simple, sensitive and reliable test suitable for high-throughput screening of Cathepsin E inhibitors. It is based on the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorescence microplate reader. at Ex/Em = 320/420 nm. In the presence of a Cathepsin E specific inhibitor, cleavage of the substrate is reduced or abolish, resulting in decrease or total loss of MCA fluorescence.
This simple and high-throughput adaptable assay kit can be used to screen, study or characterize potential inhibitors of Cathepsin E.
Cathepsin E (CTSE, EC: 22.214.171.124) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 100 tests CTSE Assay Buffer 1 x 25ml CTSE Inhibitor (1 mM Pepstatin A in DMSO) 1 x 20µl CTSE Substrate 1 x 200µl Human Cathepsin E (500 µg) 1 vial
FunctionMay have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain.
Tissue specificityExpressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B-lymphocytes.
Sequence similaritiesBelongs to the peptidase A1 family.
modificationsGlycosylated. The nature of the carbohydrate chain varies between cell types. In fibroblasts, the proenzyme contains a high mannose-type oligosaccharide, while the mature enzyme contains a complex-type oligosaccharide. In erythrocyte membranes, both the proenzyme and mature enzyme contain a complex-type oligosaccharide.
Two forms are produced by autocatalytic cleavage, form I begins at Ile-54, form II begins at Thr-57.
Cellular localizationEndosome. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome.
- Information by UniProt
- Cathepsin E
ab211082 has not yet been referenced specifically in any publications.