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  1. Link

    cathepsin-f-antibody-ab200650.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-Cathepsin F antibody (ab200650)

  • Datasheet
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  • Western blot - Anti-Cathepsin F antibody (ab200650)

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Primary

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Secondary

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Protein

 

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-Cathepsin F antibody
    See all Cathepsin F primary antibodies
  • Description

    Rabbit polyclonal to Cathepsin F
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Cathepsin F aa 261-306 (internal sequence).
    Sequence:

    MKQAKSVGDLAPPEWDWRSKGAVTKVKDQGMCGSCWAFSVTGNVEG


    Database link: Q9UBX1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • HEK293T, Raw264.7 and H9C2 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purity of ab200650 is > 95% (by SDS-PAGE).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • RAW 264.7 whole cell lysate (ab7187)
  • Recombinant Protein

    • Recombinant human Cathepsin F protein (ab157039)
  • Related Products

    • Recombinant human Cathepsin F protein (ab157039)

Applications

Our Abpromise guarantee covers the use of ab200650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.

Target

  • Function

    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • Tissue specificity

    High expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus.
  • Sequence similarities

    Belongs to the peptidase C1 family.
  • Cellular localization

    Lysosome.
  • Target information above from: UniProt accession Q9UBX1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8722 Human
    • Entrez Gene: 56464 Mouse
    • Entrez Gene: 361704 Rat
    • Omim: 603539 Human
    • SwissProt: Q9UBX1 Human
    • SwissProt: Q9R013 Mouse
    • Unigene: 11590 Human
    • Unigene: 29561 Mouse
    • Alternative names

      • AI481912 antibody
      • CATF_HUMAN antibody
      • Cathepsin F antibody
      • CathepsinF antibody
      • CATSF antibody
      • CLN13 antibody
      • Ctsf antibody
      • EC 3.4.22.41 antibody
      see all

    Images

    • Western blot - Anti-Cathepsin F antibody (ab200650)
      Western blot - Anti-Cathepsin F antibody (ab200650)
      All lanes : Anti-Cathepsin F antibody (ab200650)

      Lane 1 : HEK293T whole cell lysate
      Lane 2 : Raw264.7 whole cell lysate
      Lane 3 : H9C2 whole cell lysate

      Predicted band size: 53 kDa

    Protocols

    • Western blot protocols

    Datasheets and documents

    • Datasheet
  • References

    ab200650 has not yet been referenced specifically in any publications.

    Publishing research using ab200650? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Please use the links above to contact us or submit feedback about this product.

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