Key features and details
- Goat polyclonal to Cathepsin F
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cathepsin F antibody
See all Cathepsin F primary antibodies
DescriptionGoat polyclonal to Cathepsin F
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Dog
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab36161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.3 - 1 µg/ml. Predicted molecular weight: 53 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionThiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Tissue specificityHigh expression levels in heart, skeletal muscle, brain, testis and ovary; moderate levels in prostate, placenta, liver and colon; and no detectable expression in peripheral leukocytes and thymus.
Sequence similaritiesBelongs to the peptidase C1 family.
- Information by UniProt
- AI481912 antibody
- CATF_HUMAN antibody
- Cathepsin F antibody
Anti-Cathepsin F antibody (ab36161) at 0.3 µg/ml + Human Ovary lysate (35µg protein in RIPA buffer).
Predicted band size: 53 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
Experiments gave bands at approx 30kDa and 35kDa in Human Prostate and Ovary lysates after 0.3µg/ml antibody staining. These bands correspond to earlier findings in literature (Wang et al, J Biol Chem. 1998 Nov 27;273(48):32000-8; PMID: 9822672). This protein has a calculated MW of 53.4kDa according to NP_003784.2.
ab36161 has been referenced in 1 publication.
- Jeric B et al. N-terminally truncated forms of human cathepsin F accumulate in aggresome-like inclusions. Biochim Biophys Acta 1833:2254-66 (2013). Human . PubMed: 23684953