Overview

  • Product name

    Cathepsin G Activity Assay Kit (Colorimetric)
    See all Cathepsin G kits
  • Detection method

    Colorimetric
  • Sample type

    Serum, Tissue Extracts, Cell Lysate
  • Assay type

    Enzyme activity (quantitative)
  • Range

    0 nmol/well - 50 nmol/well
  • Assay time

    1h 00m
  • Species reactivity

    Reacts with: Mammals, Other species
  • Product overview

    Cathepsin G Activity Assay Kit (Colorimetric) (ab126780) is an assay where Cathepsin G will cleave the substrate and release the dye group, p-NA (4-Nitroaniline), which can be detected at 405 nm. In presence of the Cathepsin G specific inhibitor, the cleavage will be stopped. The kit provides a rapid, simple, sensitive, and reliable test for the activity of Cathepsin G.


    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Cathepsin G is an enzymatic protein belonging to the peptidase or protease families. The protein is found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has a specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.

  • Platform

    Microplate reader

Properties

Images

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Cathepsin G activity in THP-1 lysates (duplicates +/- SD). Data corresponds to 1 million cells per well.

  • p-NA Standard Curve. Performed according to assay protocol.
  • Assay results for 5 μl of positive control with and without an inhibitor 

Protocols

References

This product has been referenced in:

  • Hahn J  et al. Aggregated neutrophil extracellular traps resolve inflammation by proteolysis of cytokines and chemokines and protection from antiproteases. FASEB J 33:1401-1414 (2019). Read more (PubMed: 30130433) »
  • Campos MA  et al. The Biological Effects of Double-Dose Alpha-1 Antitrypsin Augmentation Therapy: A Pilot Study. Am J Respir Crit Care Med N/A:N/A (2019). Read more (PubMed: 30965011) »
See all 11 Publications for this product

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