Overview

  • Product name

    Cathepsin G Inhibitor Screening Assay Kit (Colorimetric)
    See all Cathepsin G kits
  • Detection method

    Colorimetric
  • Sample type

    Inhibitor compounds
  • Assay type

    Enzyme activity
  • Product overview

    Cathepsin G Inhibitor Screening Assay Kit (Colorimetric) (ab204693) uses the ability of active Cathepsin G to cleave a synthetic pNA (4-Nitroaniline)-based peptide substrate to release pNA, which can be easily quantified using a colorimetric microplate reader. In the presence of a Cathepsin G inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the pNA absorbance. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin G.

  • Notes

    Cathepsin G (CTSG, EC 3.4.21.20) is a Serine protease found in azurophil granules of neutrophilic polymorphonuclear leukocytes. The encoded protease has specificity similar to that of chymotrypsin C, and may participate in the killing and digestion of engulfed pathogens, and in connective tissue remodeling at sites of inflammation.

  • Platform

    Microplate reader

Properties

Images

  • Inhibition of Cathepsin G activity by CTSG Inhibitor.

Protocols

References

This product has been referenced in:

  • Goldberg EL  et al. ß-Hydroxybutyrate Deactivates Neutrophil NLRP3 Inflammasome to Relieve Gout Flares. Cell Rep 18:2077-2087 (2017). Read more (PubMed: 28249154) »
See 1 Publication for this product

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