Product nameCathepsin H overexpression 293T lysate (whole cell)
See all Cathepsin H lysates
General notesab94087 is a 293T cell transfected lysate in which Human Cathepsin H has been transiently over-expressed using a pCMV-Cathepsin H plasmid. The lysate is provided in 1X Sample Buffer. Note:For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Important for the overall degradation of proteins in lysosomes. Similarity: Belongs to the peptidase C1 family.
Our Abpromise guarantee covers the use of ab94087 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94087 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-Cathepsin H antibody (ab88683) at 1/500 dilution
Lane 1 :
Cathepsin H overexpression 293T lysate (whole cell) (ab94087)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94087 has not yet been referenced specifically in any publications.