Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
Product nameCathepsin K Activity Assay Kit (Fluorometric)
See all Cathepsin K kits
Sample typeTissue Extracts, Cell Lysate
Assay typeEnzyme activity
Assay time2h 00m
Cathepsin K Activity Assay Kit (Fluorometric) (ab65303) is a fluorescence-based assay that utilizes the preferred cathepsin K substrate sequence LR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin K will cleave the synthetic substrate LR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
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Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests CK Cell Lysis Buffer WM 1 x 25ml CK Inhibitor (1mM) Red 1 x 20µl CK Reaction Buffer NM 1 x 5ml CK Substrate Ac-LR-AFC (10mM) Amber 1 x 200µl
RelevanceCathepsin K is closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
- Cathepsin O
- Cathepsin O2
- Cathepsin X
Cathepsin K Activity measured in tissue lysates (each with protein concentration of 4 mg/mL) after 1 hour of incubation with and without inhibitor (I).
Cathepsin K Activity measured in cell lysates (each with cells concentration of 4e7 cells/mL) after 1 hour of incubation with and without inhibitor (I).
ab65303 has been referenced in 4 publications.
- Park HJ et al. 4-Phenylbutyric acid protects against lipopolysaccharide-induced bone loss by modulating autophagy in osteoclasts. Biochem Pharmacol 151:9-17 (2018). PubMed: 29458048
- Chakraborty S et al. Pasteurella multocida Toxin Triggers RANKL-Independent Osteoclastogenesis. Front Immunol 8:185 (2017). PubMed: 28289415
- Kloos B et al. Pasteurella multocida toxin- induced osteoclastogenesis requires mTOR activation. Cell Commun Signal 13:40 (2015). PubMed: 26369790
- Guery L et al. Fine-tuning nucleophosmin in macrophage differentiation and activation. Blood 118:4694-704 (2011). PubMed: 21876121