Anti-Cathepsin K antibody (ab19027)
Key features and details
- Rabbit polyclonal to Cathepsin K
- Suitable for: IHC-P, ICC/IF, IHC-Fr, WB
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cathepsin K antibody
See all Cathepsin K primary antibodies -
Description
Rabbit polyclonal to Cathepsin K -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Zebrafish -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 300-329 of Rat Cathepsin K which includes residue 321. (Peptide available as ab41720.)
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Positive control
- WB: Rat kidney tissue lysate
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3588 Cathepsin K Antibody. 3588-100 is the same size as the 100 µg size of ab19027.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab19027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (3) |
Use at an assay dependent concentration.
|
| ICC/IF | (5) |
Use at an assay dependent concentration.
|
| IHC-Fr | (2) |
Use at an assay dependent concentration.
|
| WB | (6) |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 37 kDa.
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| Notes |
|---|
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 37 kDa. |
Target
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Function
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Tissue specificity
Predominantly expressed in osteclasts (bones). -
Involvement in disease
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 1513 Human
- Entrez Gene: 13038 Mouse
- Entrez Gene: 29175 Rat
- Omim: 601105 Human
- SwissProt: P43235 Human
- SwissProt: P55097 Mouse
- SwissProt: O35186 Rat
- Unigene: 632466 Human
see all -
Alternative names
- Cathepsin K antibody
- Cathepsin O antibody
- Cathepsin O1 antibody
see all
Images
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Western blot - Anti-Cathepsin K antibody (ab19027)Anti-Cathepsin K antibody (ab19027) + Rat kidney tissue lysate
Predicted band size: 37 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (120)
ab19027 has been referenced in 120 publications.
- Kayalar E et al. Effect of salvianolic acid B on new bone formation in the orthopedically expanded suture. Angle Orthod 91:248-254 (2021). PubMed: 33367542
- Tian Y et al. Melatonin inhibits RANKL-induced osteoclastogenesis through the miR-882/Rev-erba axis in Raw264.7 cells. Int J Mol Med 47:633-642 (2021). PubMed: 33416111
- Heo SC et al. Elevated Expression of Cathepsin K in Periodontal Ligament Fibroblast by Inflammatory Cytokines Accelerates Osteoclastogenesis via Paracrine Mechanism in Periodontal Disease. Int J Mol Sci 22:N/A (2021). PubMed: 33445732
- Inagaki Y et al. Gan-Lu-Yin (Kanroin), Traditional Chinese Herbal Extracts, Reduces Osteoclast Differentiation In Vitro and Prevents Alveolar Bone Resorption in Rat Experimental Periodontitis J Clin Med 10:N/A (2021). PubMed: 33498415
- Chang Y et al. Ibudilast Mitigates Delayed Bone Healing Caused by Lipopolysaccharide by Altering Osteoblast and Osteoclast Activity. Int J Mol Sci 22:N/A (2021). PubMed: 33503906