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    cathepsin-k-antibody-ab19027.pdf

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Cardiovascular Angiogenesis Adhesion / ECM Extracellular Matrix
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Anti-Cathepsin K antibody (ab19027)

  • Datasheet
  • SDS
Reviews (17)Q&A (7)References (77)

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Western blot - Anti-Cathepsin K antibody (ab19027)

    Key features and details

    • Rabbit polyclonal to Cathepsin K
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Cathepsin K antibody [EPR19992] (ab207086)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Cathepsin K antibody
      See all Cathepsin K primary antibodies
    • Description

      Rabbit polyclonal to Cathepsin K
    • Host species

      Rabbit
    • Specificity

      ab19027 recognises cathepsin K.
    • Tested Applications & Species

      Application Species
      WB
      Rat
      See all applications and species data
    • Immunogen

      Synthetic peptide, corresponding to a region within amino acids 300-329 of Rat Cathepsin K which includes residue 321. (Peptide available as ab41720.)

    • Positive control

      • WB: Rat kidney tissue lysate

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.03% Proclin 300
      Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.015% EDTA
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Extracellular Matrix
      • Neuroscience
      • Cell Adhesion Proteins
      • Proteases
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • Other Enzymes
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Other
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteolytic enzymes
      • Cysteine protease
      • Cathepsins
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Other

    Associated products

    • Assay kits

      • Cathepsin K Activity Assay Kit (Fluorometric) (ab65303)
    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Rat Cathepsin K protein (His tag) (ab235712)
    • Related Products

      • Cathepsin K Inhibitor Assay Kit (Fluorometric) (ab185439)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab19027 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Rat
    Application Abreviews Notes
    WB (6)
    Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 37 kDa.
    Notes
    WB
    Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 37 kDa.

    Target

    • Function

      Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
    • Tissue specificity

      Predominantly expressed in osteclasts (bones).
    • Involvement in disease

      Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
    • Sequence similarities

      Belongs to the peptidase C1 family.
    • Cellular localization

      Lysosome.
    • Target information above from: UniProt accession P43235 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 29175 Rat
      • SwissProt: O35186 Rat
      • Unigene: 5598 Rat
      • Alternative names

        • Cathepsin K antibody
        • Cathepsin O antibody
        • Cathepsin O1 antibody
        • Cathepsin O2 antibody
        • Cathepsin X antibody
        • CATK_HUMAN antibody
        • CTS02 antibody
        • Ctsk antibody
        • CTSO antibody
        • CTSO1 antibody
        • CTSO2 antibody
        • MGC23107 antibody
        • PKND antibody
        • PYCD antibody
        see all

      Images

      • Western blot - Anti-Cathepsin K antibody (ab19027)
        Western blot - Anti-Cathepsin K antibody (ab19027)
        Anti-Cathepsin K antibody (ab19027) + Rat kidney tissue lysate

        Predicted band size: 37 kDa

      Protocols

      • Immunocytochemistry & immunofluorescence protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (77)

      Publishing research using ab19027? Please let us know so that we can cite the reference in this datasheet.

      ab19027 has been referenced in 77 publications.

      • Pan J  et al. Interspecies comparison of alveolar bone biology: Tooth extraction socket healing in mini pigs and mice. J Periodontol N/A:N/A (2020). PubMed: 32347546
      • Turkkahraman H  et al. Root resorption and ensuing cementum repair by Wnt/ß-catenin dependent mechanism. Am J Orthod Dentofacial Orthop N/A:N/A (2020). PubMed: 32381434
      • Sun Z  et al. Macrophage galectin-3 enhances intimal translocation of vascular calcification in diabetes mellitus. Am J Physiol Heart Circ Physiol 318:H1068-H1079 (2020). PubMed: 32216615
      • Chen CH  et al. A Novel Osteotomy Preparation Technique to Preserve Implant Site Viability and Enhance Osteogenesis. J Clin Med 8:N/A (2019). PubMed: 30717291
      • Liu W  et al. Spatial Distribution of Biomaterial Microenvironment pH and Its Modulatory Effect on Osteoclasts at the Early Stage of Bone Defect Regeneration. ACS Appl Mater Interfaces 11:9557-9572 (2019). PubMed: 30720276
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-10 of 24 Abreviews or Q&A

      Question

      ab19027 lot GR8321-5 not working. 5% milk 1/200, RT for a few hours or O/N 4°C or 2 days 4°C Human fibroblasts  

      Read More

      Abcam community

      Verified customer

      Asked on Dec 14 2011

      Answer

      Merci de nous avoir contactés et de nous avoir signalé le problème avec le ab19027. Les détails fournis sur le protocole utilisé vont nous permettre de prendre les dispositions nécessaires pour assurer une bonne qualité de nos produits. Comme convenu, j'ai mis en place l'envoi d'une unité de ab85716 en remplacement gratuit du ab19027. Le numéro de la commande est : ********. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition. Comme cet anticorps ab85716 n'a pas encore été testé en IHC, je vous invite à soumettre vos résultats sous forme d'abreview. Notre système d'Abreviews permet aux chercheurs de soumettre leur étude sur ce produit en échange de Abpoints. Les Abpoints peuvent être échangés en remise sur un prochain achat ou récompensés par des achats sur Amazon.fr. Nous pourrons, grâce à ce résultat mettre à jour la fiche technique du produit. https://www.abcam.com/abreviews N'hésitez pas à me contacter de nouveau si vous avez d'autres questions.

      Read More

      Abcam Scientific Support

      Answered on Dec 14 2011

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin K antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Vascular smooth muscle cells)
      Permeabilization
      Yes - NP40
      Specification
      Vascular smooth muscle cells
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Oct 22 2018

      Immunohistochemistry (Frozen sections) abreview for Anti-Cathepsin K antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Bone)
      Permeabilization
      Yes - Triton X
      Specification
      Bone
      Blocking step
      Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Fixative
      Paraformaldehyde
      Read More

      Dr. Tina Yuan

      Verified customer

      Submitted Jan 04 2018

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin K antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Axolotl Cell (Osteoclasts in limb section)
      Permeabilization
      Yes - Triton X-100 0.1%
      Specification
      Osteoclasts in limb section
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
      Fixative
      MEMFa
      Read More

      Mr. Camilo Riquelme Guzman

      Verified customer

      Submitted Sep 06 2017

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cathepsin K antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Mouse Tissue sections (Bone)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate
      Permeabilization
      Yes - Triton X-100
      Specification
      Bone
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Dr. Tina Yuan

      Verified customer

      Submitted Apr 24 2017

      ELISA abreview for Anti-Cathepsin K antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      ELISA
      Sample
      Mouse Recombinant protein (Recombinant, active mouse CTSK (Biovision 7597))
      Specification
      Recombinant, active mouse CTSK (Biovision 7597)
      Blocking step
      BSA as blocking agent for 2 hour(s) and 21 minute(s) · Concentration: 1% · Temperature: 25°C
      Type
      Indirect
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 01 2017

      Western blot abreview for Anti-Cathepsin K antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Vascular smooth muscle cells)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      30 µg
      Specification
      Vascular smooth muscle cells
      Blocking step
      Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 02 2016

      Western blot abreview for Anti-Cathepsin K antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (Mouse Calvarial)
      Gel Running Conditions
      Reduced Denaturing (12)
      Loading amount
      25 µg
      Treatment
      Insulin as positive control
      Specification
      Mouse Calvarial
      Blocking step
      Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 13 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin K antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Sample
      Mouse Cell (hepatocytes)
      Specification
      hepatocytes
      Permeabilization
      Yes - 0.1% Triton X-100
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 17 2014

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cathepsin K antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Blocking step
      PBS 1% BSA + 5% NGS as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: citrate
      Sample
      Mouse Tissue sections (bone)
      Specification
      bone
      Permeabilization
      No
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Mar 24 2014

      1-10 of 24 Abreviews or Q&A

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