Key features and details
- Rabbit polyclonal to Cathepsin K
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cathepsin K antibody
See all Cathepsin K primary antibodies
DescriptionRabbit polyclonal to Cathepsin K
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Non human primates
Synthetic peptide corresponding to Human Cathepsin K aa 250 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following lysates: Human Bone Marrow Tissue; Human Lung Tissue; U2OS Whole Cell; MCF7 Whole Cell; MDA-MB-231 Whole Cell; MDA-MB-361 Whole Cell; Saos 2 Whole Cell.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab85716 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 37 kDa).|
FunctionClosely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
Tissue specificityPredominantly expressed in osteclasts (bones).
Involvement in diseaseDefects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
Sequence similaritiesBelongs to the peptidase C1 family.
- Information by UniProt
- Cathepsin K antibody
- Cathepsin O antibody
- Cathepsin O1 antibody
All lanes : Anti-Cathepsin K antibody (ab85716) at 1 µg/ml
Lane 1 : Bone marrow (Human) Tissue Lysate
Lane 2 : Lung (Human) Tissue Lysate
Lane 3 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 4 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : MDA-MB-361 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 7 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
We hypothesize that the 24 kDa band represents the mature form of Cathepsin K.
ab85716 has been referenced in 1 publication.
- Maatouk L et al. TLR9 activation via microglial glucocorticoid receptors contributes to degeneration of midbrain dopamine neurons. Nat Commun 9:2450 (2018). PubMed: 29934589