The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. Predicted molecular weight: 37 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT and 1/5000 for chemiluminescent substrates.
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
Predominantly expressed in osteclasts (bones).
Involvement in disease
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
Western blot - Anti-Cathepsin K antibody - Catalytic domain (ab49893)
All lanes : Anti-Cathepsin K antibody - Catalytic domain (ab49893) at 1/1000 dilution
Lane 1 : r-Human Cathepsin K at 0.04 µg Lane 2 : r-Human Cathepsin K at 0.02 µg Lane 3 : r-Human Cathepsin K at 0.01 µg Lane 4 : r-Human Cathepsin K at 0.004 µg Lane 5 : r-Human Cathepsin K at 0.002 µg