Overview

  • Product name

    Anti-Cathepsin K antibody [EP9403]
    See all Cathepsin K primary antibodies
  • Description

    Rabbit monoclonal [EP9403] to Cathepsin K
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    within Human Cathepsin K aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P43235

  • Positive control

    • WB: Human and rat cartilage lysate, mouse and rat muscle lysates, human skeletal muscle lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab187647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).

Target

  • Function

    Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
  • Tissue specificity

    Predominantly expressed in osteclasts (bones).
  • Involvement in disease

    Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature.
  • Sequence similarities

    Belongs to the peptidase C1 family.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cathepsin K antibody
    • Cathepsin O antibody
    • Cathepsin O1 antibody
    • Cathepsin O2 antibody
    • Cathepsin X antibody
    • CATK_HUMAN antibody
    • CTS02 antibody
    • Ctsk antibody
    • CTSO antibody
    • CTSO1 antibody
    • CTSO2 antibody
    • MGC23107 antibody
    • PKND antibody
    • PYCD antibody
    see all

Images

  • All lanes : Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (purified)

    Lane 1 : Mouse cartilage lysates
    Lane 2 : Rat muscle lysates
    Lane 3 : Mouse muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 37 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?

  • All lanes : Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (Purified)

    Lane 1 : Human cartilage lysates
    Lane 2 : Human skeletal muscle lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 37 kDa
    Observed band size: 39 kDa why is the actual band size different from the predicted?

  • Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/5000 dilution (unpurified) + Rat cartilage lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.

  • Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (unpurified) + Mouse muscle lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 37 kDa
    Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.

References

ab187647 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab187647.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up