Recombinant Anti-Cathepsin K antibody [EPR24829-101] (ab300569)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)](https://www.abcam.com/ps/products/300/ab300569/Images/AB300569-6-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-mouse-kidney.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24829-101] to Cathepsin K
- Suitable for: IHC-Fr, IHC-P
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-Cathepsin K antibody [EPR24829-101]
See all Cathepsin K primary antibodies -
Description
Rabbit monoclonal [EPR24829-101] to Cathepsin K -
Host species
Rabbit -
Specificity
We do not recommend this antibody for mouse IHC-Fr.
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Tested applications
Suitable for: IHC-Fr, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse bone, rat bone, FFPE tissue sections. IHC-Fr: Rat P0 bone.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24829-101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/100.
|
|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IHC-Fr
1/100. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Tissue specificity
Predominantly expressed in osteclasts (bones). -
Involvement in disease
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 13038 Mouse
- Entrez Gene: 29175 Rat
- SwissProt: P55097 Mouse
- SwissProt: O35186 Rat
- Unigene: 272085 Mouse
- Unigene: 5598 Rat
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Alternative names
- Cathepsin K antibody
- Cathepsin O antibody
- Cathepsin O1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on mouse kidney (PMID:8647859). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)](https://www.abcam.com/ps/products/300/ab300569/Images/AB300569-5-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-rat-bone.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)Immunohistochemical analysis of paraffin-embedded rat bone tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on osteoclasts in rat bone (PMID:29322325). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)](https://www.abcam.com/ps/products/300/ab300569/Images/AB300569-4-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-mouse-bone.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)Immunohistochemical analysis of paraffin-embedded mouse bone tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on osteoclasts in mouse bone (PMID:29322325). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)](https://www.abcam.com/ps/products/300/ab300569/Images/AB300569-3-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-frozen-rat-liver.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Negative control: no staining on rat liver (PMID:25356585). is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.
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![Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)](https://www.abcam.com/ps/products/300/ab300569/Images/AB300569-2-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-frozen-rat-p0-bone.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (AB300569)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat P0 bone (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on osteoclasts of rat P0 bone is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.
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![Anti-Cathepsin K antibody [EPR24829-101] (AB300569)](https://www.abcam.com/ps/products/300/ab300569/Images/AB300569-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Cathepsin K antibody [EPR24829-101] (AB300569)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab300569 has not yet been referenced specifically in any publications.