Cathepsin K Inhibitor Screening Kit (Fluorometric) (ab185439)

Overview

  • Product name
    Cathepsin K Inhibitor Screening Kit (Fluorometric)
    See all Cathepsin K kits
  • Detection method
    Fluorescent
  • Sample type
    Inhibitor compounds
  • Assay type
    Semi-quantitative
  • Assay duration
    Multiple steps standard assay
  • Product overview

    Cathepsin K Inhibitor Screening Kit (ab185439) uses the ability of active Cathepsin K to cleave the synthetic AFC based peptide substrate torelease AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin K-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin K.

     

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  • Notes

    Cathepsin K (CTSK, EC 3.4.22.38), a lysosomal cysteine protease, is involved in osteoclastic bone remodeling and resorption. In addition, it also degrades collagen, gelatin and elastin.

  • Platform
    Microplate

Properties

  • Storage instructions
    Please refer to protocols.
  • Components 100 tests
    Cathepsin K 1 vial
    CTS K Inhibitor (FF-FMK, 1 mM) 1 x 20µl
    CTS K Reaction Buffer 1 x 15ml
    CTS K Reagent 1 x 100µl
    CTS K Substrate (Ac-LR-AFC 10 mM) 1 x 0.2ml
  • Research areas
  • Relevance
    Cathepsin K is closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
  • Cellular localization
    Lysosome.
  • Alternative names
    • Cathepsin O
    • Cathepsin O2
    • Cathepsin X
    • CTSK
    • CTSO
    • CTSO2
    see all

Associated products

Images

  • Inhibition of Cathepsin K activity by Cathepsin K Inhibitor Screening Kit was performed following kit protocol. This is example data only.

Protocols

References

ab185439 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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