Key features and details
- Assay type: Semi-quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Product nameCathepsin K Inhibitor Assay Kit (Fluorometric)
See all Cathepsin K kits
Sample typeInhibitor compounds
Cathepsin K Inhibitor Screening Kit (ab185439) uses the ability of active Cathepsin K to cleave the synthetic AFC based peptide substrate torelease AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin K-specific inhibitor, the cleavage of this substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This simple and high-throughput adaptable assay kit can be used to screen/study/characterize potential inhibitors of Cathepsin K.
Visit our FAQs page for tips and troubleshooting.
Cathepsin K (CTSK, EC 184.108.40.206), a lysosomal cysteine protease, is involved in osteoclastic bone remodeling and resorption. In addition, it also degrades collagen, gelatin and elastin.
Storage instructionsPlease refer to protocols.
Components 100 tests Cathepsin K 1 vial CTS K Inhibitor (FF-FMK, 1 mM) 1 x 20µl CTS K Reaction Buffer 1 x 15ml CTS K Reagent 1 x 100µl CTS K Substrate (Ac-LR-AFC 10 mM) 1 x 0.2ml
RelevanceCathepsin K is closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation.
- Cathepsin O
- Cathepsin O2
- Cathepsin X
ab185439 has not yet been referenced specifically in any publications.