Product nameAnti-Cathepsin L + V antibody [33/2] (HRP)
See all Cathepsin L + V primary antibodies
DescriptionMouse monoclonal [33/2] to Cathepsin L + V (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Full length native protein (purified) corresponding to Human Cathepsin L + V.
EpitopeRecognizes an epitope within amino acid residues GYGFEST (265-271 in procathepsin L and 169-175 in the mature cathepsin L molecule).
- WB: Human lung and kidney (normal) tissue lysates. IHC-P: Normal human kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% Proclin
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab197278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 38 kDa).
Abcam recommends using 3% milk as the blocking agent.
All lanes : Anti-Cathepsin L + V antibody [33/2] (HRP) (ab197278) at 1/5000 dilution
Lane 1 : Lung (Human) Tissue Lysate - adult normal tissue
Lane 2 : Human kidney tissue lysate - total protein (ab30203)
Lysates/proteins at 20 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197278 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
IHC image of Cathepsin L + V staining in a section of formalin-fixed paraffin-embedded normal human kidney*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab197278 at 1/500 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
ab197278 has not yet been referenced specifically in any publications.