Cathepsin L Activity Assay Kit (Fluorometric) (ab65306)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate
Overview
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Product name
Cathepsin L Activity Assay Kit (Fluorometric)
See all Cathepsin L kits -
Detection method
Fluorescent -
Sample type
Serum, Plasma, Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity -
Assay time
2h 00m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam's Cathepsin L Activity Assay Kit is a fluorescence-based assay that utilizes the preferred cathepsin-L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin L will cleave the synthetic substrate FR-AFC to release free AFC, which can be measured in a spectrophotometer at Em = 505 nm. The amount of free AFC will be directly proportional to the cathepsin L activity found in the sample.
Visit our FAQs page for tips and troubleshooting. -
Notes
This product is manufactured by BioVision, an Abcam company and was previously called K142 Cathepsin L Activity Fluorometric Assay Kit. K142-100 is the same size as the 100 test size of ab65306.
Cathepsin L plays a major role in antigen processing, tumor invasion and metastasis, bone resorption, and turnover of intracellular and secreted proteins involved in growth regulation. Although commonly recognized as a lysosomal protease, cathepsin L is also secreted. This broad-spectrum protease is potent in degrading several extracellular proteins (laminins, fibronectin, collagens I and IV, elastin, and other structural proteins of basement membranes) as well as serum proteins and cytoplasmic and nuclear proteins.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cathepsin Inhibitor 1 x 20µl Cathepsin L Positive Control 1 vial Cathepsin L Substrate 1 x 200µl DTT II 1 x 100µl Lysis Buffer VII 1 x 25ml -
Research areas
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Relevance
Cathepsin L is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha 1 protease inhibitor, a major controlling element of neutrophil elastase activity. Cathepsin L has been implicated in several pathologic processes, including rheumatoid arthritis, muscular dystrophy, Alzheimers, cancer, myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. Cathepsin L, which is a member of the peptidase C1 family, is a dimer composed of disulfide linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for its gene. -
Cellular localization
Lysosome -
Alternative names
- Cathepsin L1
- CathepsinL
- CATL
see all
Associated products
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Related Products
Images
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Nuclear Cathepsin L activity measured in the arcuate nucleus of: Column 1: wt lean mice, saline injected intraperitoneally; Column 2: wt fasted mice, saline injected intraperitoneally; Column 3: wt fasted mice, leptin injected intraperitoneally (1µg/g total body weight); Column 4: wt lean mice, placed in a 4C cold room for 4h; Column 5: Lepob mice, saline injected intraperitoneally; Column 6: wt lean mice, leptin and Cathepsin L inhibitor I in corn oil injected intraperitoneally (15µg/g total body weight). *,statiscally significant (p<0.01) Image obtained from Stratigopoulos G et al; JBC, 2011 Jan 21;286(3):2155-70
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Cathepsin L activity in cell lysates (duplicates +/- SD). Data from lysates corresponding to 0.5-2 million cells per well.
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Cathepsin L activity in biological fluids (serum in the range of neat-1/27 dilution, plasma neat; duplicates +/- SD)
Datasheets and documents
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SDS download
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Datasheet download
References (44)
ab65306 has been referenced in 44 publications.
- Zheng P et al. ER proteins decipher the tubulin code to regulate organelle distribution. Nature 601:132-138 (2022). PubMed: 34912111
- Dettleff P et al. Molecular Characterization of Embryos with Different Buoyancy Levels in the Yellowtail Kingfish (Seriola lalandi). Animals (Basel) 12:N/A (2022). PubMed: 35327117
- Yang WL et al. Potential drug discovery for COVID-19 treatment targeting Cathepsin L using a deep learning-based strategy. Comput Struct Biotechnol J 20:2442-2454 (2022). PubMed: 35602976
- Daura E et al. Cystatin B deficiency results in sustained histone H3 tail cleavage in postnatal mouse brain mediated by increased chromatin-associated cathepsin L activity. Front Mol Neurosci 15:1069122 (2022). PubMed: 36533126
- Romano R et al. Alteration of the late endocytic pathway in Charcot-Marie-Tooth type 2B disease. Cell Mol Life Sci 78:351-372 (2021). PubMed: 32280996