Overview

  • Product name
  • Description
    Rabbit polyclonal to Cathepsin L
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human Cathepsin L.

    (Peptide available as ab109646.)

  • Positive control
    • This antibody gave a positive signal in both HeLa and HCT116 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab95154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37, 25 kDa (predicted molecular weight: 37 kDa).

Target

  • Function
    Important for the overall degradation of proteins in lysosomes.
  • Sequence similarities
    Belongs to the peptidase C1 family.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • cathepsin L, 1 b antibody
    • Cathepsin L1 antibody
    • Cathepsin L1 light chain antibody
    • CathepsinL antibody
    • CATL antibody
    • CATL1_HUMAN antibody
    • cb15 antibody
    • CTSL antibody
    • CTSL1 antibody
    • ctsl1b antibody
    • FLJ31037 antibody
    • hgg1 antibody
    • Major excreted protein antibody
    • MEP antibody
    • MGC123162 antibody
    • wu:fb30g09 antibody
    see all

Images

  • All lanes : Anti-Cathepsin L antibody (ab95154) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HCT 116 (Human Colorectal Carcinoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa
    Additional bands at: 25 kDa (possible cleavage fragment), 35 kDa (possible cleavage fragment), 71 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 12 minutes


    The bands observed at 35 + 25 kDa could potentially be cleaved forms of Cathepsin L due to the presence of both a 17 amino acid signal peptide and a 96 amino acid activation peptide. This suggests that both the pre and mature form are observed in this blot.

References

ab95154 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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