Overview

  • Product name

    Anti-Cathepsin L/MEP antibody
    See all Cathepsin L/MEP primary antibodies
  • Description

    Rabbit polyclonal to Cathepsin L/MEP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Mouse Cathepsin L/MEP aa 80-120 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    FGDMTNEEFRQ VVNGYRHQKH KKGRLFQEPL MLKIPKSVDW


    Database link: P06797

  • Positive control

    • Rat skin tissue; Rat liver lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab203028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 37 kDa.
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200

Target

Images

  • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded rat skin tissue labeling Cathepsin L/MEP with ab203028 at 1/200 dilution with DAB staining.

  • All lanes : Anti-Cathepsin L/MEP antibody (ab203028) at 1/200 dilution

    Lane 1 : Rat liver lysate
    Lane 2 : Rat brain lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

    Predicted band size: 37 kDa

References

ab203028 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (CHO cells)
Gel Running Conditions
Reduced Denaturing (4-12%Nupage, MOPS buffer)
Loading amount
2000 cells
Specification
CHO cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 07 2018

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