Cathepsin S Activity Assay Kit (Fluorometric) (ab65307)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
Overview
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Product name
Cathepsin S Activity Assay Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Enzyme activity -
Assay time
2h 00m -
Product overview
Cathepsin S Activity Assay Kit (ab65307) is a fluorescence-based assay that uses the preferred cathepsin-S substrate sequence VVR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin-S will cleave the synthetic substrate VVR-AFC to release free AFC. The released AFC can easily be quantified using a fluorometer or fluorescence plate reader.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K144 Cathepsin S Activity Fluorometric Assay Kit. K144-100 is the same size as the 100 test size of ab65307.
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Cathepsin Inhibitor 1 x 20µl Cathepsin S Lysis Buffer 1 x 25ml Cathepsin S Assay Buffer 1 x 5ml Cathepsin S Substrate 1 x 200µl -
Research areas
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Relevance
The cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin S has been shown to be able to function as an elastase over a broad pH range in alveolar macrophages. -
Cellular localization
Lysosome -
Alternative names
- CathepsinS
- CTSS
- MGC3886
Images
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab65307 has been referenced in 20 publications.
- Karimkhanloo H et al. Circulating cathepsin S improves glycaemic control in mice. J Endocrinol 248:167-179 (2021). PubMed: 33289685
- Fu R et al. Rab27a Contributes to Cathepsin S Secretion in Lacrimal Gland Acinar Cells. Int J Mol Sci 22:N/A (2021). PubMed: 33562815
- Wu J et al. Genome-Wide Screen and Validation of Microglia Pro-Inflammatory Mediators in Stroke. Aging Dis 12:786-800 (2021). PubMed: 34094642
- Fu R et al. Cathepsin S activation contributes to elevated CX3CL1 (fractalkine) levels in tears of a Sjögren's syndrome murine model. Sci Rep 10:1455 (2020). PubMed: 31996771
- Dai Z et al. Rational Design of a Humanized Antibody Inhibitor of Cathepsin B. Biochemistry 59:1420-1427 (2020). PubMed: 32212642