Key features and details
- Rabbit polyclonal to Cathepsin S
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cathepsin S antibody
See all Cathepsin S primary antibodies
DescriptionRabbit polyclonal to Cathepsin S
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Dog
Recombinant full length protein corresponding to Human Cathepsin S aa 115-331. (Expressed in E.coli).
LPDSVDWREKGCVTEVKYQGSCGACWAFSAVGALEAQLKLKTGKLVSLSA QNLVDCSTEKYGNKGCNGGFMTTAFQYIIDNKGIDSDASYPYKAMDQKCQ YDSKYRAATCSKYTELPYGREDVLKEAVANKGPVSVGVDARHPSFFLYRS GVYYEPSCTQNVNHGVLVVGYGDLNGKEYWLVKNSWGHNFGEEGYIRMAR NKGNHCGIASFPSYPEI
Database link: P25774
- IHC-P: Human colorectal cancer, stomach cancer, stomach, kidney and glioma tissues. WB: Human lung and thymus lysates; PANC-1 cell lysate; Recombinant human Cathepsin S protein.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232830 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
FunctionThiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
Sequence similaritiesBelongs to the peptidase C1 family.
- Information by UniProt
- Cathepsin S antibody
- CathepsinS antibody
- CATS_HUMAN antibody
- Ctss antibody
Paraffin-embedded human colorectal cancer tissue stained for Cathepsin S using ab232830 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human stomach cancer tissue stained for Cathepsin S using ab232830 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human stomach tissue stained for Cathepsin S using ab232830 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human kidney tissue stained for Cathepsin S using ab232830 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human glioma tissue stained for Cathepsin S using ab232830 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Cathepsin S antibody (ab232830) at 2 µg/ml + Recombinant human Cathepsin S protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 37 kDa
All lanes : Anti-Cathepsin S antibody (ab232830) at 2 µg/ml
Lane 1 : Human lung lysate
Lane 2 : PANC-1 (human pancreatic epithelial carcinoma cell line) cell lysate
Lane 3 : Human thymus lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 37 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232830 has not yet been referenced specifically in any publications.