Recombinant
RabMAb

Recombinant Anti-Cathepsin S antibody [EPR5128] (ab134157)

Overview

  • Product name

    Anti-Cathepsin S antibody [EPR5128]
    See all Cathepsin S primary antibodies
  • Description

    Rabbit monoclonal [EPR5128] to Cathepsin S
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human Cathepsin S (UniProt P25774).

  • Positive control

    • Human spleen, U87-MG, and A549 cell lysates; Human kidney tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
    • Sequence similarities

      Belongs to the peptidase C1 family.
    • Cellular localization

      Lysosome.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cathepsin S antibody
      • CathepsinS antibody
      • CATS_HUMAN antibody
      • Ctss antibody

    Images

    • All lanes : Anti-Cathepsin S antibody [EPR5128] (ab134157) at 1/1000 dilution

      Lane 1 : Human spleen
      lysate
      Lane 2 : U87-MG cell lysate
      Lane 3 : A549 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 37 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Cathepsin S with ab134157 at 1/50 dilution.

    References

    This product has been referenced in:

    • Sun JX  et al. SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization. Angiogenesis 21:635-652 (2018). Read more (PubMed: 29675549) »
    • Erbayraktar Z  et al. Cell division cycle 7-kinase inhibitor PHA-767491 hydrochloride suppresses glioblastoma growth and invasiveness. Cancer Cell Int 16:88 (2016). WB ; Human . Read more (PubMed: 27891063) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (aorta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    No
    Specification
    aorta
    Blocking step
    No blocking step used for 10 minute(s) · Temperature: 20°C
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted May 24 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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