Product nameAnti-Cathepsin S antibody [EPR5128]
See all Cathepsin S primary antibodies
DescriptionRabbit monoclonal [EPR5128] to Cathepsin S
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Cathepsin S aa 300 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P25774
- WB: Human spleen, U87-MG, and A549 cell lysates. IHC-P: Human kidney tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134157 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 37 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
Sequence similaritiesBelongs to the peptidase C1 family.
- Information by UniProt
- Cathepsin S antibody
- CathepsinS antibody
- CATS_HUMAN antibody
- Ctss antibody
All lanes : Anti-Cathepsin S antibody [EPR5128] (ab134157) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Cathepsin S with ab134157 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This product has been referenced in:
- Sun JX et al. SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization. Angiogenesis 21:635-652 (2018). WB . Read more (PubMed: 29675549) »
- Erbayraktar Z et al. Cell division cycle 7-kinase inhibitor PHA-767491 hydrochloride suppresses glioblastoma growth and invasiveness. Cancer Cell Int 16:88 (2016). WB ; Human . Read more (PubMed: 27891063) »