Overview

  • Product name

    Cathepsin S Inhibitor Assay Kit (Fluorometric)
    See all Cathepsin S kits
  • Detection method

    Fluorescent
  • Sample type

    Inhibitor compounds
  • Assay type

    Semi-quantitative
  • Product overview

    Cathepsin S Inhibitor Screening Kit (ab185437) is an assay that uses the ability of Cathepsin S to cleave the synthetic AFC based peptide substrate to release AFC, which can be easily quantified using a fluorometer or fluorescence microplate reader. In the presence of a Cathepsin S inhibitor, the cleavage of the substrate is reduced/abolished resulting in decrease or total loss of the AFC fluorescence. This high-throughput adaptable assay kit is simple, sensitive and rapid tool to screen the potential inhibitors of Cathepsin S.

     

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  • Notes

    Cathepsin S (CTSS, EC 3.4.22.27) is a lysosomal cysteine proteinase that is suggested to participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    Cathepsin S 1 vial
    CTSS Inhibitor (Z-FF-FMK 1 mM) 1 x 20µl
    CTSS Reaction Buffer 1 x 15ml
    CTSS Substrate (Z-VVR-AFC 10 mM) 1 x 0.2ml
  • Research areas

  • Relevance

    The cathepsin family of proteases contains several diverse classes of enzymes. The cysteine protease class comprises cathepsin B, H, K, L, O, and S. The aspartyl protease class contains cathepsin D and E. Cathepsin G belongs to the serine protease class. Cathepsins are involved in various cellular events such as peptide biosynthesis, protein degradation, and apoptosis. Cathepsin S has been shown to be able to function as an elastase over a broad pH range in alveolar macrophages.
  • Cellular localization

    Lysosome
  • Alternative names

    • CathepsinS
    • CTSS
    • MGC3886

Images

  • Inhibition of Cathepsin S activity by Cathepsin S Inhibitor Screening Kit was performed following kit protocol. This is example data only.

Protocols

References

ab185437 has not yet been referenced specifically in any publications.

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