Anti-Cathepsin V antibody (ab124656)
Key features and details
- Rabbit polyclonal to Cathepsin V
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
-
Product name
Anti-Cathepsin V antibody
See all Cathepsin V primary antibodies -
Description
Rabbit polyclonal to Cathepsin V -
Host species
Rabbit -
Specificity
ab124656 detects endogenous levels of fragment of activated CATL2 resulting from cleavage adjacent to Leu114. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
A synthetic peptide, corresponding to internal sequence amino acids of Human Cathepsin V.
-
Positive control
- Human breast tissue; 293 and COS cell extracts, treated with etoposide 25uM 1h.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine)
PBS (without Mg2+, Ca2+) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Predicted molecular weight: 37 kDa.
|
|
IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/500 - 1/1000. Predicted molecular weight: 37 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Cysteine protease. May have an important role in corneal physiology. -
Tissue specificity
Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
-
Database links
- Entrez Gene: 1515 Human
- Omim: 603308 Human
- SwissProt: O60911 Human
- Unigene: 610096 Human
- Unigene: 621851 Human
-
Alternative names
- CAT L2 antibody
- Cathepsin L2 antibody
- Cathepsin U antibody
see all
Images
-
ab124656, at 1/500 dilution, staining Cathepsin V in Formalin-Fixed, Paraffin-Embedded Human breast tissue by Immunohistochemistry.
-
All lanes : Anti-Cathepsin V antibody (ab124656) at 1/500 dilution
Lane 1 : 293 cell extracts, treated with etoposide 25uM 1h
Lane 2 : COS cell extracts, treated with etoposide 25uM 1h
Lane 3 : 293 cell extracts, treated with etoposide 25uM 1h with synthesized peptide
Predicted band size: 37 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab124656 has been referenced in 2 publications.
- Panwar P et al. Expression of elastolytic cathepsins in human skin and their involvement in age-dependent elastin degradation. Biochim Biophys Acta Gen Subj 1864:129544 (2020). PubMed: 32007579
- Matsukawa S et al. Possible Association between Cathepsin V and the Development of Placenta Accreta Spectrum Disorders. Gynecol Obstet Invest 84:396-406 (2019). PubMed: 30759440