Key features and details
- Rabbit polyclonal to Cathepsin V
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cathepsin V antibody
See all Cathepsin V primary antibodies
DescriptionRabbit polyclonal to Cathepsin V
Specificityab124656 detects endogenous levels of fragment of activated CATL2 resulting from cleavage adjacent to Leu114.
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
A synthetic peptide, corresponding to internal sequence amino acids of Human Cathepsin V.
- Human breast tissue; 293 and COS cell extracts, treated with etoposide 25uM 1h.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS (without Mg2+, Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab124656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 37 kDa.|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCysteine protease. May have an important role in corneal physiology.
Tissue specificityPredominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.
Sequence similaritiesBelongs to the peptidase C1 family.
- Information by UniProt
- CAT L2 antibody
- Cathepsin L2 antibody
- Cathepsin U antibody
ab124656, at 1/500 dilution, staining Cathepsin V in Formalin-Fixed, Paraffin-Embedded Human breast tissue by Immunohistochemistry.
All lanes : Anti-Cathepsin V antibody (ab124656) at 1/500 dilution
Lane 1 : 293 cell extracts, treated with etoposide 25uM 1h
Lane 2 : COS cell extracts, treated with etoposide 25uM 1h
Lane 3 : 293 cell extracts, treated with etoposide 25uM 1h with synthesized peptide
Predicted band size: 37 kDa
ab124656 has not yet been referenced specifically in any publications.