Key features and details
- Mouse monoclonal [BV55-1] to Cathepsin V
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Cathepsin V antibody [BV55-1]
See all Cathepsin V primary antibodies
DescriptionMouse monoclonal [BV55-1] to Cathepsin V
SpecificityThis antibody recognizes human Cathepsin L2 and Procathepsin L2, but not other Cathepsins.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide surrounding amino acid 21 of human cathepsin L2.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab24508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 20 - 30 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 37 kDa.|
FunctionCysteine protease. May have an important role in corneal physiology.
Tissue specificityPredominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.
Sequence similaritiesBelongs to the peptidase C1 family.
- Information by UniProt
- CAT L2 antibody
- Cathepsin L2 antibody
- Cathepsin U antibody
All lanes : Anti-Cathepsin V antibody [BV55-1] (ab24508) at 1/1000 dilution
Lane 1 : Human hepatocyte lysate
Lane 2 : Human liver tissue lysate
Lysates/proteins at 25 µg per lane.
All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Exposure time: 28 seconds
IHC image of ab24508 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24508, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab24508 has been referenced in 2 publications.
- Jing J et al. Elevated CTSL2 expression is associated with an adverse prognosis in hepatocellular carcinoma. Int J Clin Exp Pathol 11:4035-4043 (2018). PubMed: 31949793
- Sun T et al. Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients. Oncol Lett 11:575-583 (2016). IHC . PubMed: 26870250