• Product name

    Anti-Cathepsin V antibody [BV55-1]
    See all Cathepsin V primary antibodies
  • Description

    Mouse monoclonal [BV55-1] to Cathepsin V
  • Host species

  • Specificity

    This antibody recognizes human Cathepsin L2 and Procathepsin L2, but not other Cathepsins.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide surrounding amino acid 21 of human cathepsin L2.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

  • Clone number

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab24508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 20 - 30 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 37 kDa.


  • Function

    Cysteine protease. May have an important role in corneal physiology.
  • Tissue specificity

    Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.
  • Sequence similarities

    Belongs to the peptidase C1 family.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • CAT L2 antibody
    • Cathepsin L2 antibody
    • Cathepsin U antibody
    • Cathepsin V antibody
    • CathepsinL2 antibody
    • CathepsinU antibody
    • CathepsinV antibody
    • CATL 2 antibody
    • CATL2 antibody
    • CATL2_HUMAN antibody
    • CTS L2 antibody
    • CTS U antibody
    • CTS V antibody
    • CTSL 2 antibody
    • CTSL2 antibody
    • CTSU antibody
    • CTSV antibody
    • MGC125957 antibody
    • PRO305 antibody
    • UNQ268 antibody
    see all


  • All lanes : Anti-Cathepsin V antibody [BV55-1] (ab24508) at 1/1000 dilution

    Lane 1 : Human hepatocyte lysate
    Lane 2 : Human liver tissue lysate

    Lysates/proteins at 25 µg per lane.

    All lanes : HRP-conjugated goat anti-mouse IgG polyclonal at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

    Exposure time: 28 seconds

    See Abreview

  • IHC image of ab24508 staining in human colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24508, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Sun T  et al. Expression profile of cathepsins indicates the potential of cathepsins B and D as prognostic factors in breast cancer patients. Oncol Lett 11:575-583 (2016). IHC . Read more (PubMed: 26870250) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Human Tissue lysate - nuclear (human liver, human hepatocytes)
human liver, human hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Oct 14 2014

Western blot
Human Cell lysate - whole cell (A375 cells)
Loading amount
50 µg
A375 cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Mar 03 2009


Thank you for filling out the technical help questionnaire, and for your patience. Your protocol looks correct but I have a few suggestions I would like you to try that should improve your signal. 1. If your lysis buffer does not contain protease inhibitors, I recommend you include a cocktail of various inhibitors and that you keep the lysate on ice throughout the harvest procedure to minimize proteolytic degradation. 2. Please consider loading more protein per gel lane. We recommend a minimum of 30 ug/lane. 3. Check for efficiency of transfer by staining the membrane with Ponceau Red as described in our Western blotting guide found in the Protocols section of our website. 4. To enrich for cathepsin in your samples, consider isolating the lysosomal fraction of your lysates and running this on your gel. The following reference describes a basic protocol for this: Junji Ezaki, Mitsue Takeda-Ezaki, and Eiki Kominami. Tripeptidyl Peptidase I, the Late Infantile Neuronal Ceroid Lipofuscinosis Gene Product, Initiates the Lysosomal Degradation of Subunit c of ATP Synthase; J. Biochem. Vol. 128, pp. 509-516 (2000). 5. Some customers have found that switching from milk to BSA and reducing the percentage of protein in their blocking buffer and antibody diluent to 1% or even 0.5 and 0.25 % improves the signal from a variety of antibodies. Block for no more than 1 hour at room temperature. We have not had reports of this working for this particular antibody but if you are not getting background or non-specific bands, it may be worth trying. 6. It is possible the level of cathepsin in MCF7 is below the detection limit of this antibody. The highest expression in normal tissue has been reported to be in thymus, according to the GeneCard and SwissProt links on our datasheet. We have a lysate of human adult thymus, ab30146, and many other lysates of fetal, tumor, and normal thymus fractions which can be found by searching for "thymus" using our product browser: https://www.abcam.com/index.html?datasheet=30146 I hope these suggestions help. We have not had other complaints about this antibody, but if these suggestions do not help, we will gladly arrange for a replacement or refund.

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Your complaint is the first we have received relating to this antibody. I would appreciate it if you could provide me with some further details; 1. The species of cell lysate that you have been using. 2. The method of preparation of the cell lysate. 3. The mass of protein that you have been loading. 4. The blocking agent and dilution that you have been using. 5. The secondary antibody that you have been using and its dilution. I would also appreciate details of the "control" antibody that you have been using. I appreciate your time and look forward to hearing from you.

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