Product nameAnti-Cathepsin V antibody [EPR10723(B)]
See all Cathepsin V primary antibodies
DescriptionRabbit monoclonal [EPR10723(B)] to Cathepsin V
Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to residues in Human Cathepsin V (UniProt ID: O60911).
- A549, HeLa, SW480, fetal kidney and Human small intestine lysates; A549 cells; HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab166894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 37 kDa.|
|ICC/IF||1/250 - 1/500.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionCysteine protease. May have an important role in corneal physiology.
Tissue specificityPredominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.
Sequence similaritiesBelongs to the peptidase C1 family.
- Information by UniProt
- CAT L2 antibody
- Cathepsin L2 antibody
- Cathepsin U antibody
All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution
Lane 1 : A549 lysate
Lane 2 : HeLa lysate
Lane 3 : SW480 lysate
Lane 4 : fetal kidney lysate
Lane 5 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa
Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.
Flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
ab166894 has not yet been referenced specifically in any publications.