Recombinant
RabMAb

Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)

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Overview

  • Product name
    Anti-Cathepsin V antibody [EPR10723(B)]
    See all Cathepsin V primary antibodies
  • Description
    Rabbit monoclonal [EPR10723(B)] to Cathepsin V
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human Cathepsin V (UniProt ID: O60911).

  • Positive control
    • A549, HeLa, SW480, fetal kidney and Human small intestine lysates; A549 cells; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR10723(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function
      Cysteine protease. May have an important role in corneal physiology.
    • Tissue specificity
      Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.
    • Sequence similarities
      Belongs to the peptidase C1 family.
    • Cellular localization
      Lysosome.
    • Information by UniProt
    • Database links
      • Alternative names
        • CAT L2 antibody
        • Cathepsin L2 antibody
        • Cathepsin U antibody
        • Cathepsin V antibody
        • CathepsinL2 antibody
        • CathepsinU antibody
        • CathepsinV antibody
        • CATL 2 antibody
        • CATL2 antibody
        • CATL2_HUMAN antibody
        • CTS L2 antibody
        • CTS U antibody
        • CTS V antibody
        • CTSL 2 antibody
        • CTSL2 antibody
        • CTSU antibody
        • CTSV antibody
        • MGC125957 antibody
        • PRO305 antibody
        • UNQ268 antibody
        see all

      Images

      • Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution

        Lane 1 : A549 lysate
        Lane 2 : HeLa lysate
        Lane 3 : SW480 lysate
        Lane 4 : fetal kidney lysate
        Lane 5 : Human small intestine lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 37 kDa

      • Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
      • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.
      • Flow Cytometry - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Flow Cytometry - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

      References

      ab166894 has not yet been referenced specifically in any publications.

      Publishing research using ab166894? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-Cathepsin V antibody [EPR10723(B)]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      25 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Mouse Tissue lysate - whole (liver)
      Specification
      liver
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 17 2014

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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