
Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
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Overview
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Product nameAnti-Cathepsin V antibody [EPR10723(B)]
See all Cathepsin V primary antibodies -
DescriptionRabbit monoclonal [EPR10723(B)] to Cathepsin V
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide corresponding to residues in Human Cathepsin V (UniProt ID: O60911).
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Positive control
- A549, HeLa, SW480, fetal kidney and Human small intestine lysates; A549 cells; HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR10723(B)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab166894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/1000 - 1/10000. Predicted molecular weight: 37 kDa. | |
ICC/IF | 1/250 - 1/500. | |
IP | 1/10 - 1/100. | |
Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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FunctionCysteine protease. May have an important role in corneal physiology.
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Tissue specificityPredominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.
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Sequence similaritiesBelongs to the peptidase C1 family.
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Cellular localizationLysosome.
- Information by UniProt
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Database links
- Entrez Gene: 1515 Human
- Omim: 603308 Human
- SwissProt: O60911 Human
- Unigene: 610096 Human
- Unigene: 621851 Human
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Alternative names
- CAT L2 antibody
- Cathepsin L2 antibody
- Cathepsin U antibody
see all
Images
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All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution
Lane 1 : A549 lysate
Lane 2 : HeLa lysate
Lane 3 : SW480 lysate
Lane 4 : fetal kidney lysate
Lane 5 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
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Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.
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Flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Protocols
Datasheets and documents
References
ab166894 has not yet been referenced specifically in any publications.