Recombinant
RabMAb

Recombinant Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)

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Overview

  • Product name

    Anti-Cathepsin V antibody [EPR10723(B)]
    See all Cathepsin V primary antibodies

  • Description

    Rabbit monoclonal [EPR10723(B)] to Cathepsin V

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IP, Flow Cytmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human Cathepsin V (UniProt ID: O60911).

  • Positive control

    • A549, HeLa, SW480, fetal kidney and Human small intestine lysates; A549 cells; HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10723(B)

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab166894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function

      Cysteine protease. May have an important role in corneal physiology.

    • Tissue specificity

      Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.

    • Sequence similarities

      Belongs to the peptidase C1 family.

    • Cellular localization

      Lysosome.
    • Information by UniProt

    • Database links

      • Alternative names

        • CAT L2 antibody
        • Cathepsin L2 antibody
        • Cathepsin U antibody
        • Cathepsin V antibody
        • CathepsinL2 antibody
        • CathepsinU antibody
        • CathepsinV antibody
        • CATL 2 antibody
        • CATL2 antibody
        • CATL2_HUMAN antibody
        • CTS L2 antibody
        • CTS U antibody
        • CTS V antibody
        • CTSL 2 antibody
        • CTSL2 antibody
        • CTSU antibody
        • CTSV antibody
        • MGC125957 antibody
        • PRO305 antibody
        • UNQ268 antibody
        see all

      Images

      • Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution

        Lane 1 : A549 lysate
        Lane 2 : HeLa lysate
        Lane 3 : SW480 lysate
        Lane 4 : fetal kidney lysate
        Lane 5 : Human small intestine lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 37 kDa

      • Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG.
      • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution.
      • Flow Cytometry - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Flow Cytometry - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
        Flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

      References

      This product has been referenced in:

      • Yi WJ  et al. Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage. Cell Cycle 17:844-857 (2018). Read more (PubMed: 29623762) »
      See 1 Publication for this product

      Publishing research using ab166894? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-Cathepsin V antibody [EPR10723(B)]

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      25 µg
      Gel Running Conditions
      Reduced Denaturing
      Sample
      Mouse Tissue lysate - whole (liver)
      Specification
      liver
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 17 2014

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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