Recombinant Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
![Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.com/ps/products/166/ab166894/Images/ab166894-165238-anti-cathepsin-v-antibody-epr10723b-western-blot.jpg "Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10723(B)] to Cathepsin V
- Suitable for: WB, ICC/IF, IP, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-Cathepsin V antibody [EPR10723(B)]
See all Cathepsin V primary antibodies -
Description
Rabbit monoclonal [EPR10723(B)] to Cathepsin V -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IP, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to residues in Human Cathepsin V (UniProt ID: O60911).
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Positive control
- A549, HeLa, SW480, fetal kidney and Human small intestine lysates; A549 cells; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10723(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab166894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
|
| ICC/IF |
1/250 - 1/500.
|
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| IP |
1/10 - 1/100.
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| Flow Cyt |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa. |
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ICC/IF
1/250 - 1/500. |
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IP
1/10 - 1/100. |
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Flow Cyt
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Application notes
Is unsuitable for IHC-P.
Target
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Function
Cysteine protease. May have an important role in corneal physiology. -
Tissue specificity
Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 1515 Human
- Omim: 603308 Human
- SwissProt: O60911 Human
- Unigene: 610096 Human
- Unigene: 621851 Human
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Alternative names
- CAT L2 antibody
- Cathepsin L2 antibody
- Cathepsin U antibody
see all
Images
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Western blot - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)All lanes : Anti-Cathepsin V antibody [EPR10723(B)] (ab166894) at 1/1000 dilution
Lane 1 : A549 lysate
Lane 2 : HeLa lysate
Lane 3 : SW480 lysate
Lane 4 : fetal kidney lysate
Lane 5 : Human small intestine lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
![Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.com/ps/products/166/ab166894/Images/ab166894-165237-anti-cathepsin-v-antibody-epr10723b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)Western blot analysis on immunoprecipitation pellet from Human small intestine lysate, using ab166894 at 1/1000 and HRP-conjugated anti-rabbit IgG, preferentially detecting the non-reduced form of rabbit IgG. -
![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.com/ps/products/166/ab166894/Images/ab166894-165236-anti-cathepsin-v-antibody-epr10723b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)Immunofluorescent analysis of A549 cells labeling Cathepsin V with ab166894 at 1/250 dilution. -
![Flow Cytometry - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.com/ps/products/166/ab166894/Images/ab166894-165235-anti-cathepsin-v-antibody-epr10723b-flow-cytometry.jpg)
Flow Cytometry - Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)Flow cytometric analysis of permeabilized HeLa using ab166894 at 1/10 dilution (red) or a rabbit IgG (negative) (green). -
![Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)](https://www.abcam.com/ps/products/166/ab166894/Images/ab166894-5-benefits-of-recombinant-antibodies.png)
Anti-Cathepsin V antibody [EPR10723(B)] (ab166894)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab166894 has been referenced in 1 publication.
- Yi WJ et al. Degraded melanocores are incompetent to protect epidermal keratinocytes against UV damage. Cell Cycle 17:844-857 (2018). PubMed: 29623762