Recombinant Anti-Cathepsin Z antibody [EPR14357-7] (ab182575)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14357-7] to Cathepsin Z
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cathepsin Z antibody [EPR14357-7]
See all Cathepsin Z primary antibodies -
Description
Rabbit monoclonal [EPR14357-7] to Cathepsin Z -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver and WI-38 lysate. HeLa cells and cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14357-7 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/80.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
|
IP |
1/20 - 1/40.
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Notes |
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Flow Cyt (Intra)
1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
IP
1/20 - 1/40. |
Target
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Function
Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 1522 Human
- Entrez Gene: 64138 Mouse
- Entrez Gene: 252929 Rat
- Omim: 603169 Human
- SwissProt: Q9UBR2 Human
- SwissProt: Q9WUU7 Mouse
- SwissProt: Q9R1T3 Rat
- Unigene: 252549 Human
see all -
Alternative names
- Carboxypeptidase LB antibody
- Cathepsin B2 antibody
- Cathepsin IV antibody
see all
Images
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Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/5000 dilution + WI-38 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 20 µg
Predicted band size: 34 kDa
Observed band size: 34 kDa -
Western blot analysis of Cathepsin Z in immunoprecipitation pellets from WI-38 lysate immunoprecipitated using ab182575 at 1/50 dilution.
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.
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Intracellular flow cytometric analysis of 2%paraformaldehyde-fixedHeLa cells labeling Cathepsin Z with ab182575 at 1/80 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibodyat 1/150 dilution.
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All lanes : Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/2000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HeLa lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab182575 has been referenced in 3 publications.
- Xie T et al. Insight into the Neuroprotective Effect of Genistein-3'-Sodium Sulfonate Against Neonatal Hypoxic-Ischaemic Brain Injury in Rats by Bioinformatics. Mol Neurobiol 60:807-819 (2023). PubMed: 36370154
- Wang K et al. Establishment and External Validation of a Hypoxia-Derived Gene Signature for Robustly Predicting Prognosis and Therapeutic Responses in Glioblastoma Multiforme. Biomed Res Int 2022:7858477 (2022). PubMed: 35155681
- Eichler M et al. The caspase-2 substrate p54nrb exhibits a multifaceted role in tumor cell death susceptibility via gene regulatory functions. Cell Death Dis 13:386 (2022). PubMed: 35444189