Recombinant
RabMAb

Recombinant Anti-Cathepsin Z antibody [EPR14357-7] (ab182575)

Overview

  • Product name

    Anti-Cathepsin Z antibody [EPR14357-7]
    See all Cathepsin Z primary antibodies
  • Description

    Rabbit monoclonal [EPR14357-7] to Cathepsin Z
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Cathepsin Z aa 50-200 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UBR2

  • Positive control

    • Human fetal liver and WI-38 lysate. HeLa cells and cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab182575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
Flow Cyt 1/80.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/20 - 1/40.

Target

  • Function

    Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the peptidase C1 family.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Carboxypeptidase LB antibody
    • Cathepsin B2 antibody
    • Cathepsin IV antibody
    • Cathepsin P antibody
    • Cathepsin X antibody
    • Cathepsin X precursor antibody
    • Cathepsin Y antibody
    • Cathepsin Z antibody
    • Cathepsin Z precursor antibody
    • Cathepsin Z1 antibody
    • CathepsinP antibody
    • CathepsinX antibody
    • CathepsinZ antibody
    • CATZ_HUMAN antibody
    • CTSX antibody
    • CTSZ antibody
    • Cysteine type carboxypeptidase antibody
    • FLJ17088 antibody
    • Lysosomal carboxypeptidase B antibody
    • Preprocathepsin P antibody
    • PreprocathepsinP antibody
    see all

Images

  • Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/5000 dilution + WI-38 lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 20 µg

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

  • Western blot analysis of Cathepsin Z in immunoprecipitation pellets from WI-38 lysate immunoprecipitated using ab182575 at 1/50 dilution.

    Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Cathepsin Z with ab182575 at 1/80 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • All lanes : Anti-Cathepsin Z antibody [EPR14357-7] (ab182575) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : HeLa lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa

References

ab182575 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Chinese hamster Cell lysate - whole cell (CHO cells)
Gel Running Conditions
Reduced Denaturing (4-12%Nupage, MOPS buffer)
Loading amount
2000 cells
Specification
CHO cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 27 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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