Product nameAnti-Cathepsin Z antibody [EPR14357]
See all Cathepsin Z primary antibodies
DescriptionRabbit monoclonal [EPR14357] to Cathepsin Z
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human Cathepsin Z aa 50-200. The exact sequence is proprietary.
Database link: Q9UBR2
- Human fetal liver, HeLa, K562 and WI-36 cell lysates; Human ovarian carcinoma tissue.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab180580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).|
|IHC-P||1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionExhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the peptidase C1 family.
- Information by UniProt
- Carboxypeptidase LB antibody
- Cathepsin B2 antibody
- Cathepsin IV antibody
All lanes : Anti-Cathepsin Z antibody [EPR14357] (ab180580) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : HeLa lysate
Lane 3 : K562 lysate
Lane 4 : WI-38 lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling Cathepsin Z with ab180580 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab180580 has been referenced in 1 publication.
- Aiba Y et al. Increased expression and altered localization of cathepsin Z are associated with progression to jaundice stage in primary biliary cholangitis. Sci Rep 8:11808 (2018). PubMed: 30087368