Recombinant
RabMAb

Recombinant Anti-CATSPERG antibody [EPR11608] (ab173581)

Overview

  • Product name

    Anti-CATSPERG antibody [EPR11608]
    See all CATSPERG primary antibodies
  • Description

    Rabbit monoclonal [EPR11608] to CATSPERG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, Flow Cytmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CATSPERG aa 1100 to the C-terminus (C terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q6ZRH7

  • Positive control

    • Human testis, Human seminoma, MCF-7 and Jurkat cell lysates, Human testis tissue.
  • General notes

     This product was previously labelled as C19orf15

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11608
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab173581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/5000. Predicted molecular weight: 133 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      Probably involved in sperm cell hyperactivation via its association with CATSPER1. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization.
    • Sequence similarities

      Belongs to the CATSPERG family.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • C19orf15 antibody
      • Cation channel sperm associated gamma antibody
      • Cation channel sperm-associated protein subunit gamma antibody
      • Catsper channel auxiliary subunit gamma antibody
      • Catsperg antibody
      • CTSRG_HUMAN antibody
      • DKFZp434A1022 antibody
      • FLJ46353 antibody
      see all

    Images

    • Flow cytometric analysis of permeabilized Jurkat cells labeling CATSPERG using ab173581 at 1/10 dilution  (red) or a rabbit IgG (negative) (green).

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling CATSPERG with ab173581 at 1/250 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • All lanes : Anti-CATSPERG antibody [EPR11608] (ab173581) at 1/1000 dilution

      Lane 1 : Human testis lysate
      Lane 2 : Human seminoma lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 133 kDa

    References

    ab173581 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab173581.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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