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  1. Link

    cav13-antibody-l48a9-ab85491.pdf

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Neuroscience Neurotransmission Calcium Signaling Calcium Channels L-type
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Anti-CaV1.3 antibody [L48A/9] (ab85491)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [L48A/9] (ab85491)
  • Flow Cytometry - Anti-CaV1.3 antibody [L48A/9] (ab85491)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [L48A/9] (ab85491)

Key features and details

  • Mouse monoclonal [L48A/9] to CaV1.3
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-CaV1.3 antibody [L48A/9]
    See all CaV1.3 primary antibodies
  • Description

    Mouse monoclonal [L48A/9] to CaV1.3
  • Host species

    Mouse
  • Specificity

    No cross reactivity against CaV1.2.
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Hamster
  • Immunogen

    Synthetic peptide corresponding to Rat CaV1.3 aa 859-875 (N terminal).
    Sequence:

    DNKVTIDDYQEEAEDKD


    Database link: P27732
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Rat brain normal tissue lysate - membrane extract (ab29473) can be used as a positive control in WB. Flow Cyt: SH-SY5Y cells. ICC/IF: Human differentiated iPS cells. IHC-P: Mouse backskin tissue. Human hippocampus tissue.
  • General notes

    The clone number has been updated from S48A-9 to L48A/9, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    L48A/9
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Channels
    • L-type
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Positive Controls

    • Rat brain normal tissue lysate - membrane extract (ab29473)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab85491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100.
Flow Cyt
Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Notes
IHC-P
1/100.
Flow Cyt
Use 0.5-1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
  • Tissue specificity

    Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
  • Sequence similarities

    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
  • Domain

    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q01668 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 776 Human
    • Entrez Gene: 12289 Mouse
    • Omim: 114206 Human
    • SwissProt: Q99244 Hamster
    • SwissProt: Q01668 Human
    • SwissProt: Q99246 Mouse
    • Unigene: 476358 Human
    • Unigene: 9772 Mouse
    • Alternative names

      • alpha-1 polypeptide antibody
      • CAC1D_HUMAN antibody
      • CACH3 antibody
      • CACN4 antibody
      • CACNA 1D antibody
      • Cacna1d antibody
      • CACNL1A2 antibody
      • Calcium channel antibody
      • Calcium channel L type alpha 1 polypeptide isoform 2 antibody
      • Calcium channel neuroendocrine/brain type alpha 1 subunit antibody
      • Calcium channel voltage dependent L type alpha 1 antibody
      • Calcium channel voltage dependent L type alpha 1D subunit antibody
      • Cav1.3 antibody
      • CCHL1A2 antibody
      • isoform 2 antibody
      • L type antibody
      • PASNA antibody
      • SANDD antibody
      • Voltage dependent L type calcium channel subunit alpha 1D antibody
      • Voltage gated calcium channel alpha 1 subunit antibody
      • Voltage gated calcium channel alpha subunit Cav1.3 antibody
      • Voltage gated calcium channel subunit alpha Cav1.3 antibody
      • Voltage-dependent L-type calcium channel subunit alpha-1D antibody
      • Voltage-gated calcium channel subunit alpha Cav1.3 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [L48A/9] (ab85491)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [L48A/9] (ab85491)

      ab85491 staining CaV1.3 in human hippocampus tissue by IHC-P (Bouin's fixed paraffin embedded tissue sections).

      The primary antibody was used at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.

    • Flow Cytometry - Anti-CaV1.3 antibody [L48A/9] (ab85491)
      Flow Cytometry - Anti-CaV1.3 antibody [L48A/9] (ab85491)

      Overlay histogram showing SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab85491 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab85491, 0.5 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween for 20 minutes used under the same conditions.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [L48A/9] (ab85491)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [L48A/9] (ab85491)

      ab85491 staining CaV1.3 in mouse backskin tissue by IHC-P (Bouin's fixed paraffin embedded tissue sections).

      Tissue underwent antigen retrieval using microwave in citrate buffer. The primary antibody was used at 1/100 dilution and then sections were incubated with Fluorophore conjugated goat anti mouse at 1/50 dilution.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (6)

    Publishing research using ab85491? Please let us know so that we can cite the reference in this datasheet.

    ab85491 has been referenced in 6 publications.

    • Okada T  et al. Pain induces stable, active microcircuits in the somatosensory cortex that provide a therapeutic target. Sci Adv 7:N/A (2021). PubMed: 33741588
    • Gao Y  et al. Calcium Receptor and Nitric Oxide Synthase Expression in Circular Muscle of Lower Esophagus from Patients with Achalasia. Chin Med J (Engl) 131:2882-2885 (2018). PubMed: 30511698
    • Huang JJ  et al. Functional expression of the Ca2+ signaling machinery in human embryonic stem cells. Acta Pharmacol Sin 38:1663-1672 (2017). PubMed: 28713161
    • Doshina A  et al. Cortical cells reveal APP as a new player in the regulation of GABAergic neurotransmission. Sci Rep 7:370 (2017). WB . PubMed: 28337033
    • Wang QM  et al. Isradipine attenuates MPTP-induced dopamine neuron degeneration by inhibiting up-regulation of L-type calcium channels and iron accumulation in the substantia nigra of mice. Oncotarget 8:47284-47295 (2017). WB ; Mouse . PubMed: 28521299
    • Hammarsten P  et al. High Caveolin-1 Expression in Tumor Stroma Is Associated with a Favourable Outcome in Prostate Cancer Patients Managed by Watchful Waiting. PLoS One 11:e0164016 (2016). IHC-P ; Human . PubMed: 27764093

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CaV1.3 antibody [S48A-9]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (differentiated iPS cells)
    Specification
    differentiated iPS cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.05% Tween-20
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 19 2010

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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