Overview

  • Product name

    Anti-CaV1.3 antibody [L48A/9] (FITC)
    See all CaV1.3 primary antibodies
  • Description

    Mouse monoclonal [L48A/9] to CaV1.3 (FITC)
  • Host species

    Mouse
  • Conjugation

    FITC. Ex: 493nm, Em: 528nm
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Hamster
  • Immunogen

    Recombinant fragment corresponding to Rat CaV1.3 aa 859-875. NCBI Accession No. NP_058994.1
    Sequence:

    DNKVTIDDYQEEAEDKD


    Database link: P27732

  • Positive control

    • Human hippocampus lysate, rat brain lysate.
  • General notes

    The clone number has been updated from S48A-9 to L48A/9, both clone numbers name the same antibody clone.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab183405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 250 kDa.

1 μg/mL of ab183405 was sufficient to detect CaV1.3 in 10 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.

Target

  • Function

    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
  • Tissue specificity

    Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
  • Sequence similarities

    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
  • Domain

    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • alpha-1 polypeptide antibody
    • CAC1D_HUMAN antibody
    • CACH3 antibody
    • CACN4 antibody
    • CACNA 1D antibody
    • Cacna1d antibody
    • CACNL1A2 antibody
    • Calcium channel antibody
    • Calcium channel L type alpha 1 polypeptide isoform 2 antibody
    • Calcium channel neuroendocrine/brain type alpha 1 subunit antibody
    • Calcium channel voltage dependent L type alpha 1 antibody
    • Calcium channel voltage dependent L type alpha 1D subunit antibody
    • Cav1.3 antibody
    • CCHL1A2 antibody
    • isoform 2 antibody
    • L type antibody
    • PASNA antibody
    • SANDD antibody
    • Voltage dependent L type calcium channel subunit alpha 1D antibody
    • Voltage gated calcium channel alpha 1 subunit antibody
    • Voltage gated calcium channel alpha subunit Cav1.3 antibody
    • Voltage gated calcium channel subunit alpha Cav1.3 antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1D antibody
    • Voltage-gated calcium channel subunit alpha Cav1.3 antibody
    see all

References

ab183405 has not yet been referenced specifically in any publications.

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