Product nameAnti-CaV1.3 antibody [N38/8]
See all CaV1.3 primary antibodies
DescriptionMouse monoclonal [N38/8] to CaV1.3
SpecificityNo cross-reactivity against Cav1.2
Tested applicationsSuitable for: IP, ICC/IF, Flow Cyt, IHC-P, WB, Agglutination, IHC-Frmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Human
Fusion protein corresponding to C terminal amino acids 2025-2161 of Rat Cav1.3
- Rat brain lysate IHC-P: Mouse brain tissue.
At Abcam we are committed to ensuring that our customers have access to the complete dataset available for our products to allow you to select the best product for your research needs. With this in mind, for this product we highlight the fact that it is known by two different clone numbers. It's primary clone number is N38/8, but you may also see it referred to as S38-8 in literature.
The clone number has been updated from S38-8 to N38/8, both clone numbers name the same antibody clone.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab84811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 245 kDa.|
|Agglutination||Use 1-2µg for 106 cells.|
FunctionVoltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
Tissue specificityExpressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
Sequence similaritiesBelongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
DomainEach of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
- Information by UniProt
- alpha-1 polypeptide antibody
- CAC1D_HUMAN antibody
- CACH3 antibody
Frozen mouse brain tissue stained for CaV1.3 using ab84811 at 1/1000 dilution in immunohistochemical analysis.
Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at for 5 minutes at room temperature.
All lanes : Anti-CaV1.3 antibody [N38/8] (ab84811) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Rat tissue lysate
Predicted band size: 245 kDa
ab84811 staining CaV1.3 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
Overlay histogram showing SH-SY5Y cells stained with ab84811 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84811, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab84811 has not yet been referenced specifically in any publications.