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  1. Link

    cav13-antibody-n388-ab84811.pdf

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Neuroscience Neurotransmission Calcium Signaling Calcium Channels L-type
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Anti-CaV1.3 antibody [N38/8] (ab84811)

  • Datasheet
Reviews (4)Q&A (1)References (1)

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Immunohistochemistry (Frozen sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)
  • Western blot - Anti-CaV1.3 antibody [N38/8] (ab84811)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)
  • Flow Cytometry - Anti-CaV1.3 antibody [N38/8] (ab84811)

Key features and details

  • Mouse monoclonal [N38/8] to CaV1.3
  • Suitable for: Flow Cyt, IHC-P, WB, IHC-Fr
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CaV1.3 antibody [N38/8]
    See all CaV1.3 primary antibodies
  • Description

    Mouse monoclonal [N38/8] to CaV1.3
  • Host species

    Mouse
  • Specificity

    No cross-reactivity against Cav1.2
  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein corresponding to Rat CaV1.3 aa 2000-2200 (C terminal).
    Database link: P27732

  • Positive control

    • Rat brain lysate IHC-P: Mouse brain tissue.
  • General notes

    At Abcam we are committed to ensuring that our customers have access to the complete dataset available for our products to allow you to select the best product for your research needs. With this in mind, for this product we highlight the fact that it is known by two different clone numbers. It's primary clone number is N38/8, but you may also see it referred to as S38-8 in literature.

    The clone number has been updated from S38-8 to N38/8, both clone numbers name the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N38/8
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Channels
    • L-type
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Channels
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab84811 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 245 kDa.
IHC-Fr (3)
1/1000.
Notes
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 245 kDa.
IHC-Fr
1/1000.

Target

  • Function

    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA).
  • Tissue specificity

    Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle.
  • Sequence similarities

    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily.
  • Domain

    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q01668 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 776 Human
    • Entrez Gene: 12289 Mouse
    • Entrez Gene: 29716 Rat
    • Omim: 114206 Human
    • SwissProt: Q01668 Human
    • SwissProt: Q99246 Mouse
    • SwissProt: P27732 Rat
    • Unigene: 476358 Human
    • Unigene: 9772 Mouse
    • Unigene: 89671 Rat
    see all
  • Alternative names

    • alpha-1 polypeptide antibody
    • CAC1D_HUMAN antibody
    • CACH3 antibody
    • CACN4 antibody
    • CACNA 1D antibody
    • Cacna1d antibody
    • CACNL1A2 antibody
    • Calcium channel antibody
    • Calcium channel L type alpha 1 polypeptide isoform 2 antibody
    • Calcium channel neuroendocrine/brain type alpha 1 subunit antibody
    • Calcium channel voltage dependent L type alpha 1 antibody
    • Calcium channel voltage dependent L type alpha 1D subunit antibody
    • Cav1.3 antibody
    • CCHL1A2 antibody
    • isoform 2 antibody
    • L type antibody
    • PASNA antibody
    • SANDD antibody
    • Voltage dependent L type calcium channel subunit alpha 1D antibody
    • Voltage gated calcium channel alpha 1 subunit antibody
    • Voltage gated calcium channel alpha subunit Cav1.3 antibody
    • Voltage gated calcium channel subunit alpha Cav1.3 antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1D antibody
    • Voltage-gated calcium channel subunit alpha Cav1.3 antibody
    see all

Images

  • Immunohistochemistry (Frozen sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Immunohistochemistry (Frozen sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)

    Frozen mouse brain tissue stained for CaV1.3 using ab84811 at 1/1000 dilution in immunohistochemical analysis.

    Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at for 5 minutes at room temperature.

  • Western blot - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Western blot - Anti-CaV1.3 antibody [N38/8] (ab84811)
    All lanes : Anti-CaV1.3 antibody [N38/8] (ab84811) at 1 µg/ml

    Lane 1 : Molecular weight marker
    Lane 2 : Rat tissue lysate

    Predicted band size: 245 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)
    ab84811 staining CaV1.3 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • Flow Cytometry - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Flow Cytometry - Anti-CaV1.3 antibody [N38/8] (ab84811)
    Overlay histogram showing SH-SY5Y cells stained with ab84811 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84811, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (1)

Publishing research using ab84811? Please let us know so that we can cite the reference in this datasheet.

ab84811 has been referenced in 1 publication.

  • Gao Y  et al. The anesthetic bupivacaine induces cardiotoxicity by targeting L-type voltage-dependent calcium channels. J Int Med Res 48:300060520942619 (2020). PubMed: 32812463

Customer reviews and Q&As

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1-4 of 4 Abreviews

Immunohistochemistry (Frozen sections) abreview for Anti-CaV1.3 antibody [S38-8]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (atrium)
Specification
atrium
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Apr 25 2012

Immunohistochemistry (Frozen sections) abreview for Anti-CaV1.3 antibody [S38-8]

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (heart)
Specification
heart
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 18 2012

Immunohistochemistry (Frozen sections) abreview for Anti-CaV1.3 antibody [S38-8]

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X 100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jan 23 2012

Immunocytochemistry abreview for Anti-CaV1.3 antibody [S38-8]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Rabbit Cell (atrial cell)
Specification
atrial cell
Fixative
Paraformaldehyde
Permeabilization
Yes - triton-X
Read More

Abcam user community

Verified customer

Submitted Jan 20 2012

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