Anti-CaV1.3 antibody [N38/8] (ab84811)
![Immunohistochemistry (Frozen sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)](https://www.abcam.com/ps/products/84/ab84811/Images/ab84811-310694-anti-cav13-antibody-n38-8-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)")
Key features and details
- Mouse monoclonal [N38/8] to CaV1.3
- Suitable for: Flow Cyt, IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-CaV1.3 antibody [N38/8]
See all CaV1.3 primary antibodies -
Description
Mouse monoclonal [N38/8] to CaV1.3 -
Host species
Mouse -
Specificity
No cross-reactivity against Cav1.2 -
Tested applications
Suitable for: Flow Cyt, IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Fusion protein corresponding to Rat CaV1.3 aa 2000-2200 (C terminal).
Database link: P27732 -
Positive control
- Rat brain lysate IHC-P: Mouse brain tissue.
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General notes
At Abcam we are committed to ensuring that our customers have access to the complete dataset available for our products to allow you to select the best product for your research needs. With this in mind, for this product we highlight the fact that it is known by two different clone numbers. It's primary clone number is N38/8, but you may also see it referred to as S38-8 in literature.
The clone number has been updated from S38-8 to N38/8, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N38/8 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab84811 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
|
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| IHC-P |
Use at an assay dependent concentration.
|
|
| WB |
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 245 kDa.
|
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| IHC-Fr | (3) |
1/1000.
|
| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 245 kDa. |
|
IHC-Fr
1/1000. |
Target
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Function
Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1D gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). -
Tissue specificity
Expressed in pancreatic islets and in brain, where it has been seen in cerebral cortex, hippocampus, basal ganglia, habenula and thalamus. Expressed in the small cell lung carcinoma cell line SCC-9. No expression in skeletal muscle. -
Sequence similarities
Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1D subfamily. -
Domain
Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 776 Human
- Entrez Gene: 12289 Mouse
- Entrez Gene: 29716 Rat
- Omim: 114206 Human
- SwissProt: Q01668 Human
- SwissProt: Q99246 Mouse
- SwissProt: P27732 Rat
- Unigene: 476358 Human
see all -
Alternative names
- alpha-1 polypeptide antibody
- CAC1D_HUMAN antibody
- CACH3 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)
Frozen mouse brain tissue stained for CaV1.3 using ab84811 at 1/1000 dilution in immunohistochemical analysis.
Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at room temperature. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at for 5 minutes at room temperature.
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![Western blot - Anti-CaV1.3 antibody [N38/8] (ab84811)](https://www.abcam.com/ps/products/84/ab84811/Images/ab84811-99536-anti-cav13-antibody-n38-8-western-blot.jpg)
Western blot - Anti-CaV1.3 antibody [N38/8] (ab84811)All lanes : Anti-CaV1.3 antibody [N38/8] (ab84811) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Rat tissue lysate
Predicted band size: 245 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)](https://www.abcam.com/ps/products/84/ab84811/Images/ab84811-99537-anti-cav13-antibody-n38-8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaV1.3 antibody [N38/8] (ab84811)ab84811 staining CaV1.3 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded). -
![Flow Cytometry - Anti-CaV1.3 antibody [N38/8] (ab84811)](https://www.abcam.com/ps/products/84/ab84811/Images/ab84811-99535-anti-cav13-antibody-n38-8-flow-cytometry.jpg)
Flow Cytometry - Anti-CaV1.3 antibody [N38/8] (ab84811)Overlay histogram showing SH-SY5Y cells stained with ab84811 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84811, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab84811 has been referenced in 1 publication.
- Gao Y et al. The anesthetic bupivacaine induces cardiotoxicity by targeting L-type voltage-dependent calcium channels. J Int Med Res 48:300060520942619 (2020). PubMed: 32812463
