Product nameAnti-Caveolin-1 antibody
See all Caveolin-1 primary antibodies
DescriptionGoat polyclonal to Caveolin-1
SpecificityNo cross-reactivity expected to Caveolin-2 and Caveolin-3.
Tested applicationsSuitable for: ICC/IF, IHC-FoFr, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
- Human spleen lysate. Lysate from transfected HEK293 cells transiently expressing Caveolin-1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab36152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. See Abreviews.|
|IHC-FoFr||1/100. PubMed: 18716216|
|WB||Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa).|
FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Tissue specificityExpressed in muscle and lung, less so in liver, brain and kidney.
Involvement in diseaseDefects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Sequence similaritiesBelongs to the caveolin family.
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
Cellular localizationGolgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
- Information by UniProt
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
Anti-Caveolin-1 antibody (ab36152) at 0.3 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer).
Predicted band size: 20 kDa
Observed band size: 21,25 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
All lanes : Anti-Caveolin-1 antibody (ab36152) at 1 µg/ml
Lane 1 : HEK293 cell lysate overexpressing Caveolin 1
Lane 2 : MOCK-transfected HEK293 cell lysate
Predicted band size: 20 kDa
Observed band size: 23 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Cuddapah VA et al. Calcium entry via TRPC1 channels activates chloride currents in human glioma cells. Cell Calcium 53:187-94 (2013). Read more (PubMed: 23261316) »
- Hunt CL et al. The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus. J Virol 85:334-47 (2011). WB ; Human . Read more (PubMed: 21047970) »