Anti-Caveolin-1 antibody (ab36152)
Key features and details
- Goat polyclonal to Caveolin-1
- Suitable for: Flow Cyt (Intra), ICC, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Caveolin-1 antibody
See all Caveolin-1 primary antibodies -
Description
Goat polyclonal to Caveolin-1 -
Host species
Goat -
Specificity
No cross-reactivity expected to Caveolin-2 and Caveolin-3. -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human Caveolin-1 aa 33-44 (internal sequence).
Sequence:DELSEKQVYDAH
Database link: Q03135 -
Positive control
- ICC: U-2 OS and A431 cells; WB: A549 and A431 cell lysates. Human heart tissue lysate. Flow Cyt (Intra): HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
Dog
Pig
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
|
| ICC |
Use a concentration of 10 µg/ml.
|
|
| WB | (3) |
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa).
1 hour primary incubation is recommended for this product. |
| Notes |
|---|
|
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
|
ICC
Use a concentration of 10 µg/ml. |
|
WB
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
Tissue specificity
Expressed in muscle and lung, less so in liver, brain and kidney. -
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
Sequence similarities
Belongs to the caveolin family. -
Post-translational
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 403980 Dog
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 404693 Pig
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: P33724 Dog
- SwissProt: Q03135 Human
see all -
Alternative names
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
Images
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Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)
Immunocytochemistry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells labeling Caveolin-1 with ab36152 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL.
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Western blot - Anti-Caveolin-1 antibody (ab36152)Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml + Human Heart lysate at 35 µg
Predicted band size: 20 kDa -
Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody (ab36152)Flow Cytometry (Intracellular) analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Caveolin-1 with ab36152 at 10 µg/mL (blue line). Cells permeabilized with 0.5% Triton. Primary incubation overnight followed by Alexa Fluor® 488 secondary antibody at 1 µg/mL.
IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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Western blot - Anti-Caveolin-1 antibody (ab36152)Lane 1 : Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml
Lane 2 : Anti-Caveolin-1 antibody (ab36152) at 0.001 µg/ml
Lane 1 : A431 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 20 kDa -
Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling Caveolin-1 with ab36152 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL.
Protocols
Datasheets and documents
References (6)
ab36152 has been referenced in 6 publications.
- Takagaki Y et al. Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice. Sci Rep 9:7519 (2019). PubMed: 31101909
- Lenis TL et al. Expression of ABCA4 in the retinal pigment epithelium and its implications for Stargardt macular degeneration. Proc Natl Acad Sci U S A 115:E11120-E11127 (2018). PubMed: 30397118
- Stucki JD et al. Medium throughput breathing human primary cell alveolus-on-chip model. Sci Rep 8:14359 (2018). PubMed: 30254327
- Cuddapah VA et al. Calcium entry via TRPC1 channels activates chloride currents in human glioma cells. Cell Calcium 53:187-94 (2013). PubMed: 23261316
- Hunt CL et al. The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus. J Virol 85:334-47 (2011). WB ; Human . PubMed: 21047970
- Schmidtko A et al. cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I. J Neurosci 28:8568-76 (2008). IHC-FoFr ; Mouse . PubMed: 18716216
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