Anti-Caveolin-1 antibody (ab36152)
- Datasheet
- Specific References (3)
- Protocols
Overview
-
Product nameAnti-Caveolin-1 antibody
See all Caveolin-1 primary antibodies -
DescriptionGoat polyclonal to Caveolin-1
-
Host speciesGoat
-
SpecificityNo cross-reactivity expected to Caveolin-2 and Caveolin-3.
-
Tested applicationsSuitable for: ICC/IF, IHC-FoFr, WBmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
-
Immunogen
Synthetic peptide corresponding to Human Caveolin-1 aa 33-44 (internal sequence).
Sequence:DELSEKQVYDAH
Database link: Q03135 -
Positive control
- Human spleen lysate. Lysate from transfected HEK293 cells transiently expressing Caveolin-1.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 -
Concentration information loading... -
PurityImmunogen affinity purified
-
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab36152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. See Abreviews. | |
| IHC-FoFr | 1/100. PubMed: 18716216 | |
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa). |
Target
-
FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
-
Tissue specificityExpressed in muscle and lung, less so in liver, brain and kidney.
-
Involvement in diseaseDefects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
-
Sequence similaritiesBelongs to the caveolin family.
-
Post-translational
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
Cellular localizationGolgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
- Information by UniProt
-
Database links
- Entrez Gene: 403980 Dog
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 404693 Pig
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: P33724 Dog
- SwissProt: Q03135 Human
see all -
Alternative names
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
Images
-
Western blot - Anti-Caveolin-1 antibody (ab36152)Anti-Caveolin-1 antibody (ab36152) at 0.3 µg/ml + Human Spleen lysate (35µg protein in RIPA buffer).
Predicted band size: 20 kDa
Observed band size: 21,25 kDa (why is the actual band size different from the predicted?)
Primary incubation was 1 hour. Detected by chemiluminescence. -
Western blot - Anti-Caveolin-1 antibody (ab36152)All lanes : Anti-Caveolin-1 antibody (ab36152) at 1 µg/ml
Lane 1 : HEK293 cell lysate overexpressing Caveolin 1
Lane 2 : MOCK-transfected HEK293 cell lysate
Predicted band size: 20 kDa
Observed band size: 23 kDa (why is the actual band size different from the predicted?)
Protocols
References
This product has been referenced in:
- Cuddapah VA et al. Calcium entry via TRPC1 channels activates chloride currents in human glioma cells. Cell Calcium 53:187-94 (2013). Read more (PubMed: 23261316) »
- Hunt CL et al. The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus. J Virol 85:334-47 (2011). WB ; Human . Read more (PubMed: 21047970) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"