Anti-Caveolin-1 antibody (ab36152)
- Datasheet
- References (6)
- Protocols
Overview
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Product name
Anti-Caveolin-1 antibody
See all Caveolin-1 primary antibodies -
Description
Goat polyclonal to Caveolin-1 -
Host species
Goat -
Specificity
No cross-reactivity expected to Caveolin-2 and Caveolin-3. -
Tested applications
Suitable for: ICC/IF, IHC-FoFr, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Pig
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Immunogen
Synthetic peptide corresponding to Human Caveolin-1 aa 33-44 (internal sequence).
Sequence:DELSEKQVYDAH
Database link: Q03135 -
Positive control
- Human spleen lysate. Lysate from transfected HEK293 cells transiently expressing Caveolin-1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituent: 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab36152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. See Abreviews. | |
| IHC-FoFr | 1/100. PubMed: 18716216 | |
| WB | Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
Tissue specificity
Expressed in muscle and lung, less so in liver, brain and kidney. -
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
Sequence similarities
Belongs to the caveolin family. -
Post-translational
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 403980 Dog
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 404693 Pig
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: P33724 Dog
- SwissProt: Q03135 Human
see all -
Alternative names
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
Images
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Western blot - Anti-Caveolin-1 antibody (ab36152)Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml + Human Heart lysate at 35 µg
Predicted band size: 20 kDa -
Western blot - Anti-Caveolin-1 antibody (ab36152)Lane 1 : Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml
Lane 2 : Anti-Caveolin-1 antibody (ab36152) at 0.001 µg/ml
All lanes : A431 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 20 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Takagaki Y et al. Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice. Sci Rep 9:7519 (2019). Read more (PubMed: 31101909) »
- Lenis TL et al. Expression of ABCA4 in the retinal pigment epithelium and its implications for Stargardt macular degeneration. Proc Natl Acad Sci U S A 115:E11120-E11127 (2018). Read more (PubMed: 30397118) »
