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    caveolin-1-antibody-ab36152.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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Anti-Caveolin-1 antibody (ab36152)

  • Datasheet
Reviews (10) Submit a question References (6)

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Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)
  • Western blot - Anti-Caveolin-1 antibody (ab36152)
  • Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody (ab36152)
  • Western blot - Anti-Caveolin-1 antibody (ab36152)
  • Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)

Key features and details

  • Goat polyclonal to Caveolin-1
  • Suitable for: Flow Cyt (Intra), ICC, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Caveolin-1 antibody
    See all Caveolin-1 primary antibodies
  • Description

    Goat polyclonal to Caveolin-1
  • Host species

    Goat
  • Specificity

    No cross-reactivity expected to Caveolin-2 and Caveolin-3.
  • Tested Applications & Species

    Application Species
    ICC
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Caveolin-1 aa 33-44 (internal sequence).
    Sequence:

    DELSEKQVYDAH


    Database link: Q03135
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC: U-2 OS and A431 cells; WB: A549 and A431 cell lysates. Human heart tissue lysate. Flow Cyt (Intra): HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituent: 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Recombinant Human Caveolin-1 protein (ab114170)
  • Recombinant Protein

    • Recombinant Human Caveolin-1 protein (ab114170)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab36152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC
Human
WB
Human
All applications
Mouse
Rat
Dog
Pig
Application Abreviews Notes
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
ICC
Use a concentration of 10 µg/ml.
WB (3)
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa).

1 hour primary incubation is recommended for this product.

Notes
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
ICC
Use a concentration of 10 µg/ml.
WB
Use a concentration of 0.001 - 0.01 µg/ml. Detects a band of approximately 21, 25 kDa (predicted molecular weight: 20 kDa).

1 hour primary incubation is recommended for this product.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Target information above from: UniProt accession Q03135 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 403980 Dog
    • Entrez Gene: 857 Human
    • Entrez Gene: 12389 Mouse
    • Entrez Gene: 404693 Pig
    • Entrez Gene: 25404 Rat
    • Omim: 601047 Human
    • SwissProt: P33724 Dog
    • SwissProt: Q03135 Human
    • SwissProt: P49817 Mouse
    • SwissProt: Q6RVA9 Pig
    • SwissProt: P41350 Rat
    • Unigene: 74034 Human
    • Unigene: 28278 Mouse
    • Unigene: 470537 Mouse
    • Unigene: 22518 Rat
    see all
  • Alternative names

    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Images

  • Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)
    Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)

    Immunocytochemistry analysis of paraformaldehyde fixed A431 (human epidermoid carcinoma cell line) cells labeling Caveolin-1 with ab36152 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL.

  • Western blot - Anti-Caveolin-1 antibody (ab36152)
    Western blot - Anti-Caveolin-1 antibody (ab36152)
    Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml + Human Heart lysate at 35 µg

    Predicted band size: 20 kDa

  • Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody (ab36152)
    Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody (ab36152)

    Flow Cytometry (Intracellular) analysis of paraformaldehyde fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Caveolin-1 with ab36152 at 10 µg/mL (blue line). Cells permeabilized with 0.5% Triton. Primary incubation overnight followed by Alexa Fluor® 488 secondary antibody at 1 µg/mL.

    IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.

  • Western blot - Anti-Caveolin-1 antibody (ab36152)
    Western blot - Anti-Caveolin-1 antibody (ab36152)
    Lane 1 : Anti-Caveolin-1 antibody (ab36152) at 0.003 µg/ml
    Lane 2 : Anti-Caveolin-1 antibody (ab36152) at 0.001 µg/ml

    Lane 1 : A431 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 20 kDa

  • Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)
    Immunocytochemistry - Anti-Caveolin-1 antibody (ab36152)

    Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells labeling Caveolin-1 with ab36152 at 10 µg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL.

Protocols

  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (6)

    Publishing research using ab36152? Please let us know so that we can cite the reference in this datasheet.

    ab36152 has been referenced in 6 publications.

    • Takagaki Y  et al. Dipeptidyl peptidase-4 plays a pathogenic role in BSA-induced kidney injury in diabetic mice. Sci Rep 9:7519 (2019). PubMed: 31101909
    • Lenis TL  et al. Expression of ABCA4 in the retinal pigment epithelium and its implications for Stargardt macular degeneration. Proc Natl Acad Sci U S A 115:E11120-E11127 (2018). PubMed: 30397118
    • Stucki JD  et al. Medium throughput breathing human primary cell alveolus-on-chip model. Sci Rep 8:14359 (2018). PubMed: 30254327
    • Cuddapah VA  et al. Calcium entry via TRPC1 channels activates chloride currents in human glioma cells. Cell Calcium 53:187-94 (2013). PubMed: 23261316
    • Hunt CL  et al. The Tyro3 receptor kinase Axl enhances macropinocytosis of Zaire ebolavirus. J Virol 85:334-47 (2011). WB ; Human . PubMed: 21047970
    • Schmidtko A  et al. cGMP produced by NO-sensitive guanylyl cyclase essentially contributes to inflammatory and neuropathic pain by using targets different from cGMP-dependent protein kinase I. J Neurosci 28:8568-76 (2008). IHC-FoFr ; Mouse . PubMed: 18716216

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-10 of 10 Abreviews or Q&A

    Western blot abreview for Anti-Caveolin-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (Cardiac tissue)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8-12% Bis-Tris)
    Loading amount
    100 µg
    Specification
    Cardiac tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 10 2021

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (hiPSc derived cardiomyocyte)
    Permeabilization
    Yes - 0.02% Triton X
    Specification
    hiPSc derived cardiomyocyte
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 26 2021

    Western blot abreview for Anti-Caveolin-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart tissue)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (8-12% Bis-Tris)
    Loading amount
    50 µg
    Specification
    Heart tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 25 2021

    Immunoprecipitation abreview for Anti-Caveolin-1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Mouse Tissue lysate - whole (heart)
    Total protein in input
    1000 µg
    Immuno-precipitation step
    Other - dynabeads
    Specification
    heart
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 19 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caveolin-1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Retinal Müller Glia)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton X100 (0.1%)
    Specification
    Retinal Müller Glia
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 29 2019

    Immunohistochemistry (Frozen sections) abreview for Anti-Caveolin-1 antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Blood-Brain Barrier)
    Permeabilization
    Yes - PBS 0.1% TritonX
    Specification
    Blood-Brain Barrier
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 28 2019

    Western blot abreview for Anti-Caveolin-1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Dog Cell lysate - whole cell (MDCK)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (4-12%)
    Loading amount
    15 µg
    Specification
    MDCK
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 25 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin 1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human umbilical vein endothelial cells)
    Specification
    Human umbilical vein endothelial cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Saponine 0,1%
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Mr. Cyril Mougin

    Verified customer

    Submitted Apr 16 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin 1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (Bovine aortic endothelial cells)
    Specification
    Bovine aortic endothelial cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Saponin 0,1%
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Mr. Cyril Mougin

    Verified customer

    Submitted Apr 08 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin 1 antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (A7r5 (rat smooth muscle cells))
    Specification
    A7r5 (rat smooth muscle cells)
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Saponine 0,1%
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Mr. Cyril Mougin

    Verified customer

    Submitted Mar 24 2010

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