Overview

  • Product name
    Anti-Caveolin-1 antibody - Caveolae Marker
    See all Caveolin-1 primary antibodies
  • Description
    Rabbit polyclonal to Caveolin-1 - Caveolae Marker
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human, Pig
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Mouse Caveolin-1 aa 1-100 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab18607, ab18607, ab18607, ab18607, ab18607)

  • Positive control
    • Recombinant Human Caveolin-1 protein (ab114170) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Heart (Mouse) Tissue Lysate Heart (Human) Tissue Lysate Heart (Rat) Tissue Lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab18199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

  • Function
    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity
    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease
    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities
    Belongs to the caveolin family.
  • Post-translational
    modifications
    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization
    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • Database links
  • Alternative names
    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Images

  • All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml

    Lane 1 : Mouse Heart Lysate
    Lane 2 : Human Heart Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG (700) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab18199) at 1 µg/ml

    Lane 1 : NIH 3T3 whole cell lysate (ab7179)
    Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Caveolin 1 - Caveolae Marker was immunoprecipitated using 0.5mg Mouse Heart whole tissue lysate, 5µg of Rabbit polyclonal to Caveolin 1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Heart whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab18199.
    Secondary: Clean blot (HRP conjugate) at 1/1000 dilution.
    Band: 20kDa: Caveolin 1 - Caveolae Marker.
  • Ab18199 recognizes the full length tagged recombinant Caveolin 1 protein (ab114170) which has an expected molecular weight of 46 kDa.
  • ICC/IF image of ab18199 stained NIH/3T3 cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab18199, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • ab18199 staining human HT1080 cells by ICC/IF.  Cells were PFA fixed and permeabilized in Triton X-100 prior to incubating with ab18199 at 10 µg/ml for 1 hour at 20°C.  A Texas Red® conjugated donkey anti-rabbit antibody was used as the secondary.

    See Abreview

  • ICC/IF image of ab18199 stained MEF7cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab18199, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Cehofski LJ  et al. Dexamethasone intravitreal implant downregulates PDGFR-a and upregulates caveolin-1 in experimental branch retinal vein occlusion. Exp Eye Res 171:174-182 (2018). WB ; Pig . Read more (PubMed: 29505751) »
  • Halamoda-Kenzaoui B  et al. The agglomeration state of nanoparticles can influence the mechanism of their cellular internalisation. J Nanobiotechnology 15:48 (2017). WB ; Human . Read more (PubMed: 28651541) »
See all 18 Publications for this product

Customer reviews and Q&As

1-10 of 18 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lung)
Loading amount
25 µg
Specification
Lung
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

Submitted Dec 10 2012

Answer

Thank you for your reply.

Having talked to my colleagues we can go ahead and replace those antibodies for you; however before we do we would like to understand why there was such a delay in the package being delivered to you, so it does not happen again.

As we talked about on the telephone yesterday, according to FedEx records they tried to deliver it twice on Monday (24th Sep) & twice on Tuesday (25th), before successfully being able to deliver it on Wednesday (26th). The reason for the failed delivery attempts was that there was nobody there to receive it. Therefore I wanted to check the shipping address with you and see if it needs to go to a different location to guarantee that someone will be there Mon-Fri, 9am-5pm. The shipping address we have is:



Please could you confirm this is the correct address where the package will be sent and also please let me know if there are any days that the package should not be delivered and I will update our notes.

I look forward to your reply.

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Answer

Thank you for calling Abcam earlier today.

I am sorry that ab18199 was not working for you. I am going to talk to my colleagues about the best way to proceed win this matter.

I just had a few follow up questions:

1 - Did you try all 3 vials of the antibody? If so, did they all fail to give a signal?

2 - Could you please let me know primary antibody concentrations you have tried and also the incubation times?

I look forward to your reply and helping to resolve this issue.

Read More

Answer

Gracias por contactarnos de nuevo.

El anticuerpo Anti-Caveolin 1 (ab18199) ha sido testado en inmunoprecipitación, Western Blot e immunohistoquímica en secciones embebidas en parafina. Al haberse validado en estas aplicaciones el anticuerpo está garantizado para funcionar en tejidos de rata, y en caso de no hacerlo la garantía cubriría el reemplazo o reembolso del anticuerpo.

Como controles positivos se recomienda utilizar los siguientes lisados: NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate Heart (Mouse) Tissue Lysate Heart (Human) Tissue Lysate Heart (Rat) Tissue Lysate.

Podéis encontrar más información sobre su perfil de expresión en el siguiente link:

http://www.proteinatlas.org/ENSG00000105974

En el que tejidos de placenta, hígado y páncreas tienen un perfil moderado de expresión de Caveolin 1. Otros tejidos enfermos, como melanoma o cáncer de cuello expresan intensamente la misma.



En cuanto al anticuerpo Anti-Nrf2 (ab31163) se ha validado en las siguientes aplicaciones y por tanto está garantizado para funcionar en ellas: IHC-P, ICC/IF, IHC-Fr, WB. Se recomienda usar tejido de placenta como control, cerebro, o tracto digestivo:

http://www.proteinatlas.org/ENSG00000116044



Por último, para el anticuerpo Anti-Keap1 (ab66620), las aplicaciones testadas y garantizadas son las siguientes: ICC/IF, WB, IHC-P. Controles positivos recomendados: lisado de HepG2 y tejido de hígado humano. Más información sobre su expresión la encontrareis:

http://www.proteinatlas.org/ENSG00000079999



Si alguno de los anticuerpos no estuviera testado en la aplicación que os interesa, podéis participar en el sistema de evaluación de anticuerpos Abreview. Mediante este sistema nos mostráis vuestro interés en testar el anticuerpo en una especie o aplicación no testada y os emitimos un código canjeable por un anticuerpo gratuito.

A cambio, vosotros una vez testado el anticuerpo en la nueva aplicación publicáis una reseña en nuestra web (de forma anónima o no), independientemente del resultado obtenido. En ese momento el código enviado se activa, y podéis proceder con el nuevo pedido gratuito.

Para conocer con más detalle nuestro sistema de Abreview, visita la página: https://www.abcam.com/abreviews

Espero que esta información te sea de utilidad, en caso contrario, por favor, no dudes en volver a contactarnos.

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Answer

Gracias por contactarnos.

El producto ab66620 tiene un precio de €380.00 y actualmente en nuestra oficina de Inglaterra queda una unidad es stock. Los productos ab31163 y ab18199 valen €410.00 cada uno y tenemos suficientes unidades en stock de ambos. Si realizarais hoy el pedido antes de las 17 horas, y siguieran todos en stock, recibiríais los productos mañana o el viernes como muy tarde.

En este momento además tenemos una promoción para pedidos españoles por la que al comprar tres productos, el tercero sale a mitad de precio. Para consultar dicha promoción y su aplicabilidad, visitad el siguiente enlace:

https://www.abcam.com/index.html?pageconfig=resource&rid=15058

No olvidéis indicar el código de la promoción (50%-MAYO-X71W2) cuando hagas el pedido mandándonos un correo a mailto:orders@abcam.com.

ab66620 es válido para trabajar en rata mediante immunohistoquímica en secciones de parafina, Western Blot e Inmunocitoquímica. ab31163 y ab18199 son validos para las aplicaciones mencionadas (IP, IHC y WB), aunque ab18199 solo lo hemos testado en immunohistoquímica en secciones de parafina, no sabemos su aplicabilidad para secciones congeladas.

En cualquier caso os recuerdo que todos nuestros productos tienen una garantía (Abpromise) de 6 meses, y en caso de no funcionar como se especifica en su datasheet, se os reemplazará el producto de forma gratuita o se reembolsará el importe del mismo.

Respecto a los controles positivos, os animo a que llevéis a cabo la búsqueda de los mismos en nuestra web, pues según la aplicación y las necesidades especificas serán más convenientes unos productos que otros. Para realizar la búsqueda, en el buscador de la parte de arriba de nuestra página web, introduce la proteína, el lisado o el tejido, y en el desplegable al lado elegid la opción “Lysate” para lisados celulares para usar en WB, “slides” para secciones de tejidos, etc…Al validar la búsqueda aparecerán todas las opciones disponibles, que pueden filtrarse seleccionando las categorías de la parte izquierda de la pagina.

Si necesitáis un presupuesto, mas información sobre los productos identificados, o tenéis cualquier otra consulta, por favor, no dudéis en contactarnos de nuevo, por mail por teléfono en el 91 114 65 54.

Read More

Answer

Thank you very much for your interest in Abcam antibodies.

For most of the antibodies we have in our catalog it is possible to know the immunogen sequence or at least the immunogenic region and compare it with the sequence of interest.

For example, we know that the anti-caveolin ab18199 shows 76% homology with the chicken protein (SwissProt P35431).

If you want to test this antibody for exampleI can offer a discount off a future purchase if you buy ab18199 now, test it inChicken and submit feedback to us in the form of an Abreview.It doesn’t matter whether theAbreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test an Abcam antibody in Chicken. I will then send a discount code. This code must be issued before purchasing the antibody you would like to test, so please wait for my reply before ordering.

2. Purchasethe antibodyeither by phone, fax, or online (www.abcam.com).

3. Test it in in Chicken.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe tested antibodyturns out to be unsuitable for Chicken, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

If you are interested in this offer, please inform us which application you would like the antibody in so I can narrow down the choice of the antibodies.

I am going to contact the lab where the anti-eIF2 alpha(ab115822) is produced in order to ask for the immunogen sequence or region.

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Answer

Thank you for contacting us.

Here is the information I have received from our lab:

We haven’t tested this ab for crossreactivity with caveolin 2 and caveolin 3 but I’ve done a quick blast of our immunogen sequence against Mouse and it looks to be specific to caveolin 1.


If it does turn out that the KO mice are fine and it is our antibody, it would be wonderful if youcould let us know. In that case, please provide us with the experimental details and results, so that I can pass it on to our lab.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -80C may be viable for up to 10 years. I hope this information helps. Please contact us with any other questions.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (OVA lung)
Specification
OVA lung
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Jun 06 2011

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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