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    caveolin-1-antibody-caveolae-marker-ab2910.pdf

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Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

  • Datasheet
  • SDS
Reviews (55)Q&A (10)References (151)

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Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunoprecipitation - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

Key features and details

  • Rabbit polyclonal to Caveolin-1 - Caveolae Marker
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Caveolin-1 antibody - Caveolae Marker
    See all Caveolin-1 primary antibodies
  • Description

    Rabbit polyclonal to Caveolin-1 - Caveolae Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Cat, Pig, Chimpanzee, Gorilla, African green monkey, African bush elephant
  • Immunogen

    Synthetic peptide corresponding to Human Caveolin-1 aa 1-100.
    Database link: Q03135

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Human lung, heart and spleen tissue lysates; Mouse heart and lung tissue lysates; Rat heart protein extract; PANC-1, U-87 MG, A549, HeLa, PC-3, U-2 OS, C2C12 and A431 cell lysates. ICC/IF: A-375, HeLa and NIH/3T3 cells; Rat astrocytes.
  • General notes

    This antibody can be used as a marker for lipid raft fractions.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Caveolin-1 protein (ab114170)
  • Recombinant Protein

    • Recombinant Human Caveolin-1 protein (ab114170)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (22)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
ICC/IF (15)
Use at an assay dependent concentration.
IP (9)
Use at an assay dependent concentration.

Recommended dilution: 5 µg

Notes
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Recommended dilution: 5 µg

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Target information above from: UniProt accession Q03135 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 493668 Cat
    • Entrez Gene: 463670 Chimpanzee
    • Entrez Gene: 281040 Cow
    • Entrez Gene: 101125469 Gorilla
    • Entrez Gene: 857 Human
    • Entrez Gene: 12389 Mouse
    • Entrez Gene: 404693 Pig
    • Entrez Gene: 100008837 Rabbit
    • Entrez Gene: 25404 Rat
    • Entrez Gene: 445468 Sheep
    • Omim: 601047 Human
    • SwissProt: A0M8S7 Cat
    • SwissProt: Q2QLF2 Chimpanzee
    • SwissProt: P79132 Cow
    • SwissProt: Q2IBF0 Gorilla
    • SwissProt: Q2QLB0 Horse
    • SwissProt: Q03135 Human
    • SwissProt: P49817 Mouse
    • SwissProt: Q6RVA9 Pig
    • SwissProt: Q09YN6 Rabbit
    • SwissProt: P41350 Rat
    • SwissProt: Q6B3Y2 Sheep
    • Unigene: 74034 Human
    • Unigene: 28278 Mouse
    • Unigene: 470537 Mouse
    • Unigene: 22518 Rat
    see all
  • Alternative names

    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Images

  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 1.5 µg/ml

    Lane 1 : Human lung
    Lane 2 : Human heart
    Lane 3 : Human spleen

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

    ab2910 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab2910 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunoprecipitation - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunoprecipitation - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

    Caveolin 1 was immunoprecipitated using 5 µg of ab2910 from lysate of Mouse Heart (Lane 3) using the Dynabeads® Protein A Immunoprecipitation Kit. Normal Rabbit IgG was used as a Isotype control (Lane 2). 10 % input represents the cell extract used for immunoprecipitation (Lane 1). Western blot analysis was performed using Caveolin 1 Rabbit Polyclonal Antibody and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.



    All lanes :

    Lane 1 : Input control
    Lane 2 : Isotype control
    Lane 3 : IP elute
  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 1 µg/ml

    All lanes : CAV1 knockout HeLa cell lysate

    Predicted band size: 20 kDa



    The specificity of ab2910 was demonstrated by CRISPR targeted CAV1 knockout in HeLa cells. Western blot analysis of whole cell lysates using this antibody showed no detection of caveolin 1 protein expression in knockout cells compared to the protein detected at ~22kDa in wild-type HeLa cells. 

    Knockout validation info.
  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 1 µg/ml

    Lane 1 : PANC-1 (Human pancreatic epithelial cancinoma cell line) whole cell lysate
    Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 4 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 5 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 6 : U-2 OS (Human bone osteosarcoma epithelial cell line) whole cell lysate
    Lane 7 : Mouse heart tissue lysate
    Lane 8 : Rat heart tissue lysate
    Lane 9 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 10 : Mouse lung tissue lysate
    Lane 11 : A-431 (Human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 20 kDa



    Western blot analysis was performed on whole cell extracts (20 µg lysate). The blots were probed with Anti-ab2910 (1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. A 17 kDa band corresponding to Caveolin-1 was observed across cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 2 µg/ml + Rat heart protein extract

    Predicted band size: 20 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

    Immunofluorescence analysis of Caveolin 1 was done on 70% confluent log phase A-375 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ab2910 at 1 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

    Rat astrocytes stained with fluorescently labeled Caveolin-1 antibody.

    Primary antibody is ab2910 at a dilution of 1/500 and the secondary antibody is Texas red labeled anti-rabbit IgG at a dilution of 1/1000.

    This image was kindly supplied as part of the review submitted by Donghui Zhu.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)This image is courtesy of an anonymous Abreview

    ab2910 staining Caveolin-1 - Caveolae Marker in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.

    See Abreview

Protocols

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (151)

Publishing research using ab2910? Please let us know so that we can cite the reference in this datasheet.

ab2910 has been referenced in 151 publications.

  • Zayed MA  et al. CEPT1-Mediated Phospholipogenesis Regulates Endothelial Cell Function and Ischemia-Induced Angiogenesis Through PPARa. Diabetes 70:549-561 (2021). PubMed: 33214136
  • Busch C  et al. Type of culture medium determines properties of cultivated retinal endothelial cells: induction of substantial phenotypic conversion by standard DMEM. Heliyon 7:e06037 (2021). PubMed: 33521368
  • Li X  et al. Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes. Front Cell Dev Biol 9:640667 (2021). PubMed: 33816490
  • Hernaiz-Llorens M  et al. Growth cone repulsion to Netrin-1 depends on lipid raft microdomains enriched in UNC5 receptors. Cell Mol Life Sci 78:2797-2820 (2021). PubMed: 33095273
  • Lee LL  et al. Cardiac pericytes function as key vasoactive cells to regulate homeostasis and disease. FEBS Open Bio 11:207-225 (2021). PubMed: 33135334
View all Publications for this product

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1-10 of 55 Abreviews

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Dopaminergic Neurons)
Gel Running Conditions
Reduced Denaturing (NuPage 4-12% MES buffer)
Loading amount
30 µg
Specification
Dopaminergic Neurons
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 20 2020

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
kidney
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 13 2020

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Fractions from mouse liver tissue to obtain lipid)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
50 µg
Specification
Fractions from mouse liver tissue to obtain lipid
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 18 2019

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (human Alveolar Epithelial cells (hAELVi))
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
1e+006 cells
Specification
human Alveolar Epithelial cells (hAELVi)
Blocking step
Western Breeze Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Submitted Apr 30 2018

Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human Alveolar Epithelial cells (hAELVi))
Permeabilization
Yes - Saponin
Specification
human Alveolar Epithelial cells (hAELVi)
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 19 2018

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
50 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Juan Rodríguez Vita

Verified customer

Submitted Mar 27 2017

Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3 fibroblasts)
Gel Running Conditions
Reduced Denaturing (15%)
Loading amount
10 µg
Specification
3T3 fibroblasts
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Mar 01 2017

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Goat Tissue sections (Myocardium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Myocardium
Blocking step
(agent) for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Carl Hobbs

Verified customer

Submitted Jan 28 2016

Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (neuron)
Permeabilization
Yes - 0.2%Triton
Specification
neuron
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 13 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (COS-7)
Permeabilization
Yes - 0.2%Triton
Specification
COS-7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Ya Zhou

Verified customer

Submitted Oct 06 2015

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