Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
- Datasheet
- References (103)
- Protocols
Overview
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Product name
Anti-Caveolin-1 antibody - Caveolae Marker
See all Caveolin-1 primary antibodies -
Description
Rabbit polyclonal to Caveolin-1 - Caveolae Marker -
Host species
Rabbit -
Specificity
Detects caveolin-1. -
Tested applications
Suitable for: ICC/IF, IHC-Fr, IP, WB, IHC-P, Dot blotmore details -
Species reactivity
Reacts with: Mouse, Rat, Goat, Hamster, Dog, Human
Predicted to work with: Sheep, Rabbit, Horse, Cow, Cat, Pig, Chimpanzee, Gorilla, African green monkey, African bush elephant
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Immunogen
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Positive control
- WB: Human lung, heart and spleen lysate. Rat heart protein extract. ICC/IF: NIH/3T3 and HeLa cells. Rat astrocytes. IHC-P: Human cardiac tissue. IP: A549 cell lysate.
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General notes
This antibody can be used as a marker for lipid raft fractions.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab2910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/500. PubMed: 17502389 | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 20351069 | |
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928). | |
| IHC-P | Use at an assay dependent concentration. | |
| Dot blot | Use at an assay dependent concentration. PubMed: 25025521 |
Target
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Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
Tissue specificity
Expressed in muscle and lung, less so in liver, brain and kidney. -
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
Sequence similarities
Belongs to the caveolin family. -
Post-translational
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 493668 Cat
- Entrez Gene: 463670 Chimpanzee
- Entrez Gene: 281040 Cow
- Entrez Gene: 403980 Dog
- Entrez Gene: 101125469 Gorilla
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 404693 Pig
see all -
Alternative names
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)Image is courtesy of an AbReview submitted by Carl Hobbs.Immunohistochemical analysis of formaldehyde-fixed paraffin-embedded human cardiac tissue, labeling Caveolin 1 with ab2910 at a dilution of 1/10,000 incubated for 2 hours at 21°C in TBS / BSA / Aside solution.
Heat mediated antigen retrival was performed with citric acid. Blocking was with 1% BSA incubated for 10 minutes at 21°C. Secondary used was a goat anti-rabbit polyclonal biotin conjugate at 1/300. Image shows strong immunopositivity at the membranes of cardiomyocytes (invaginations of immunostaining show points of branching of each myocyte). There is intense positivity in the smooth muscle of of arterioles. Not seen is the intense positivity of what seems to be the endothelial lining cells of the endocardium.
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Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)ab2910 at a 1/250 dilution staining NIH/3T3 (Mouse embryo fibroblast cell line) cells by Immunocytochemistry (panel B).
The antibody was incubated with the paraformaldehyde fixed cells for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated Goat anti-rabbit antibody (ab150080). Panel A shows staining with a mouse anti-Caveolin 1 antibody (clone 2297). Panel C shows the merged image.
This image is courtesy of an Abreview by William Ackerman.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)Vicente et al PLoS One. 2018 Jun 11;13(6):e0198822. doi: 10.1371/journal.pone.0198822. eCollection 2018. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Cross-sections of normal human corneas (A, F, K) and naïve ARK corneas (B, G, L), KLAL ARK cornea (C, H, M), centered transplanted ARK cornea (D, I, N) and decentered transplanted ARK cornea (E, J, O) labeled with antibodies (green) against Ki-67 (A-E), CD68 (F-J) and caveolin-1 (ab2910) (K-O).
The corneal buttons were routinely formalin-fixed, embedded into paraffin wax and serial sections, 4 μm thick, were collected on Superfrost® Plus slides, dried in a vertical position overnight at 60°C and thereafter stored at +4°C in a tightly closed slide box until further processing. Two normal corneas were processed and sectioned using the same protocol. For full method, please see paper.
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Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 1.5 µg/ml
Lane 1 : Human lung
Lane 2 : Human heart
Lane 3 : Human spleen
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)Rat astrocytes stained with fluorescently labeled Caveolin-1 antibody.
Primary antibody is ab2910 at a dilution of 1/500 and the secondary antibody is Texas red labeled anti-rabbit IgG at a dilution of 1/1000.
This image was kindly supplied as part of the review submitted by Donghui Zhu.
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Dot Blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)Ferraz-Nogueira et al PLoS One. 2014 Jul 15;9(7):e102526. doi: 10.1371/journal.pone.0102526. eCollection 2014. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Structure and characterization of pmPAS, a plasma membrane FRET biosensor for PA.
Cells expressing pmPAS were lysed and subjected to sucrose gradient ultracentrifugation to check the localization of pmPAS in subdomains of the plasma membrane.
Fractions were probed for caveolin-1 using ab2910.
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Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)This image is courtesy of an anonymous Abreviewab2910 staining Caveolin-1 - Caveolae Marker in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.
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Immunoprecipitation - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)This image is courtesy of an anonymous Abreviewab2910 diluted 1/500 and was incubated with A549 (Human lung carcinoma cell line) whole cell lysate and a Protein A/G matrix for 16 hours at 4°C to achieve immunoprecipitation. 400 µg of lysate was present in the input.
An HRP-conjugated goat anti-rabbit was used for the Western Blot step.
Lane 1: Whole cell
Lane 2: IP-Caveolin-1
Lane 3: Unrelated antibody
Lane 4: Unrelated antibody
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Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 2 µg/ml + Rat heart protein extract
Predicted band size: 20 kDa
Protocols
References
This product has been referenced in:
- Cai X et al. miR-124a enhances therapeutic effects of bone marrow stromal cells transplant on diabetic nephropathy-related epithelial-to-mesenchymal transition and fibrosis. J Cell Biochem 121:299-312 (2020). Read more (PubMed: 31190436) »
- Zhang Z et al. Metabolic reprogramming of normal oral fibroblasts correlated with increased glycolytic metabolism of oral squamous cell carcinoma and precedes their activation into carcinoma associated fibroblasts. Cell Mol Life Sci N/A:N/A (2019). Read more (PubMed: 31270582) »
