Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-Caveolin-1 antibody [E249] - Caveolae Marker
See all Caveolin-1 primary antibodies -
DescriptionRabbit monoclonal [E249] to Caveolin-1 - Caveolae Marker
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Host speciesRabbit
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SpecificityThis antibody should recognize both alpha and beta form of Caveolin-1.
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Tested applicationsSuitable for: ICC/IF, IHC-P, ICC, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Caveolin-1 aa 150-250. The exact sequence is proprietary.
Database link: Q03135 -
Positive control
- WB: A431 cell lysate. ICC/IF: A431 cells. IHC-P: Human urinary bladder and lung tissues; Rat colon tissue; Mouse testis tissue.
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General notes
A trial size is available to purchase for this antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityIgG fraction
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ClonalityMonoclonal
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Clone numberE249
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab32577 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 19808975 | |
| IHC-P | 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 0.01M Sodium Citrate Buffer, pH 6.0. | |
| ICC | 1/100. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 20 kDa. |
Target
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FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
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Tissue specificityExpressed in muscle and lung, less so in liver, brain and kidney.
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Involvement in diseaseDefects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
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Sequence similaritiesBelongs to the caveolin family.
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Post-translational
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
Cellular localizationGolgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
- Information by UniProt
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Database links
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 25404 Rat
- Omim: 601047 Human
- SwissProt: Q03135 Human
- SwissProt: P49817 Mouse
- SwissProt: P41350 Rat
- Unigene: 74034 Human
see all -
Alternative names
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
Images
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![Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)](https://www.abcam.com/ps/products/32/ab32577/Images/ab32577wb.jpg)
Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) + A431 cell lysate
Predicted band size: 20 kDa
Observed band size: 23 kDa (why is the actual band size different from the predicted?)
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)](https://www.abcam.com/ps/products/32/ab32577/Images/ab32577-308582-ab32577IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling Caveolin-1 with Purified ab32577 at 1:500 dilution (2.1 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)](https://www.abcam.com/ps/products/32/ab32577/Images/ab32577-308577-ab32577IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling Caveolin-1 with purified ab32577 at 1:500 dilution (2.1 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)](https://www.abcam.com/ps/products/32/ab32577/Images/ab32577-288690-ab32577iccif.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)Immunocytochemistry/Immunofluorescence analysis of A431 cells labelling Caveolin-1 (green) with ab32577 at 1/200. Cells were fixed with 100% methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889 (red), an Alexa Fluor® 488 conjugated mouse anti-tubulin antibody (1/200). Nuclei counterstained with DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)](https://www.abcam.com/ps/products/32/ab32577/Images/Caveolin-1-Primary-antibodies-ab32577-1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human urinary bladder tissue, staining Caveolin-1 with ab32577 at 1/250 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)](https://www.abcam.com/ps/products/32/ab32577/Images/Caveolin-1-Primary-antibodies-ab32577-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung tissue, staining Caveolin-1 with ab32577 at 1/250 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)](https://www.abcam.com/ps/products/32/ab32577/Images/Caveolin-1-Primary-antibodies-ab32577-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)Image from Kaarteenaho R et al., BMC Dev Biol. 2010 Nov 16;10:113. Fig 6.; doi:10.1186/1471-213X-10-113; 16 November 2010, BMC Developmental Biology 2010, 10:113Immunohistochemical analysis of developing Human lung tissue, staining Caveolin-1 with ab32577 at 1/250 dilution.
References
This product has been referenced in:
- Marsboom G et al. Aberrant caveolin-1-mediated Smad signaling and proliferation identified by analysis of adenine 474 deletion mutation (c.474delA) in patient fibroblasts: a new perspective on the mechanism of pulmonary hypertension. Mol Biol Cell 28:1177-1185 (2017). Read more (PubMed: 28468941) »
- Hu L et al. Emodin and rhein decrease levels of hypoxia-inducible factor-1a in human pancreatic cancer cells and attenuate cancer cachexia in athymic mice carrying these cells. Oncotarget 8:88008-88020 (2017). Read more (PubMed: 29152137) »
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