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    caveolin-1-antibody-e249-caveolae-marker-ab32577.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

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Reviews (2)Q&A (1)References (15)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Flow Cytometry - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E249] to Caveolin-1 - Caveolae Marker
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free HRP

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Overview

  • Product name

    Anti-Caveolin-1 antibody [E249] - Caveolae Marker
    See all Caveolin-1 primary antibodies
  • Description

    Rabbit monoclonal [E249] to Caveolin-1 - Caveolae Marker
  • Host species

    Rabbit
  • Specificity

    This antibody should recognize both alpha and beta form of Caveolin-1.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431, A549 and HeLa cell lysates. ICC/IF: HeLa and Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E249
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab185043)
    • Alexa Fluor® 647 Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab193892)
    • HRP Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab193893)
    • Anti-Caveolin-1 antibody [E249] - BSA and Azide free (ab230262)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CAV1 (Caveolin-1) knockout HeLa cell line (ab255371)
    • Human CAV1 (Caveolin-1) knockout A-431 cell line (ab269583)
  • KO cell lysates

    • Human CAV1 (Caveolin-1) knockout HeLa cell lysate (ab263806)
    • Human CAV1 (Caveolin-1) knockout A-431 cell lysate (ab270706)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Caveolin-1 protein (ab114170)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use 0.01M Sodium Citrate Buffer, pH 6.0.

For unpurified use at 1:250.

ICC/IF
1/50.
Flow Cyt
1/20.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.
Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use 0.01M Sodium Citrate Buffer, pH 6.0.

For unpurified use at 1:250.

ICC/IF
1/50.
Flow Cyt
1/20.
WB
1/1000 - 1/10000. Predicted molecular weight: 20 kDa.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Target information above from: UniProt accession Q03135 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 857 Human
    • Entrez Gene: 12389 Mouse
    • Entrez Gene: 25404 Rat
    • Omim: 601047 Human
    • SwissProt: Q03135 Human
    • SwissProt: P49817 Mouse
    • SwissProt: P41350 Rat
    • Unigene: 74034 Human
    • Unigene: 28278 Mouse
    • Unigene: 470537 Mouse
    • Unigene: 22518 Rat
    see all
  • Alternative names

    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue sections labelling Caveolin-1 with purified ab32577 at 1/2000 dilution (0.06 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labelling Caveolin-1 with purified ab32577 at 1/2000 dilution (0.06 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labelling Caveolin-1 with purified ab32577 at 1/2000 dilution (0.06 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    All lanes : Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) at 1/1000 dilution

    Lane 1 : Wild-type A431 cell lysate
    Lane 2 : CAV1 knockout A431 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 20 kDa
    Observed band size: 20 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32577 observed at 20 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab32577 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. A431 wild-type and CAV1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab32577 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

    ab32577 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab32577 at 1/200 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Flow Cytometry - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Flow Cytometry - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

    Flow cytometric analysis of 4% Paraformaldehyde fixed 90% Methanol permeabilized A431 (Human epidermoid carcinoma epithelial cell) cells labelling Caveolin-1 with ab32577 at 1/20 dilution (10 µg/ml) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

  • Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    All lanes : Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : Wild-type HeLa cell lysate
    Lane 4 : CAV1 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 20 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab32577 observed at 20 kDa. Red - loading control, ab8245 observed at 37 kDa.  

     ab32577 was shown to react with Caveolin-1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab255371 (knockout cell lysate ab263806) was used. Wild-type and Caveolin-1 knockout samples were subjected to SDS-PAGE. ab32577 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    All lanes :

    Lane 1 : Mouse heart lysate
    Lane 2 : Mouse skeletal muscle lysate
    Lane 3 : Rat heart lysate
    Lane 4 : Rat skeletal muscle lysate
    Lane 5 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 17, 23 kDa why is the actual band size different from the predicted?



    The two isoforms of Caveolin-1 have been described in the literatures (PMID: 12816877, 11748292 and 14992406). We are unsure about the nature of the 250kDa bands. 

  • Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Western blot - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    All lanes : Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577) at 1/1000 dilution (Purified)

    Lane 1 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 3 : HaCaT (Human skin keratinocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 20 kDa
    Observed band size: 17, 23 kDa why is the actual band size different from the predicted?



    The two isoforms of Caveolin-1 have been described in the literatures (PMID: 12816877, 11748292 and 14992406). We are unsure about the nature of the 250kDa bands.

  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

    Immunocytochemistry analysis of Jurkat (Human lung carcinoma epithelial cell) cells labeling Caveolin-1 with purified ab32577 at 1/50 dilution (2.3 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150078) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
    Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (15)

Publishing research using ab32577? Please let us know so that we can cite the reference in this datasheet.

ab32577 has been referenced in 15 publications.

  • Sántha P  et al. Morphological Heterogeneity in Pancreatic Cancer Reflects Structural and Functional Divergence. Cancers (Basel) 13:N/A (2021). PubMed: 33672734
  • Chen Y  et al. Irradiation pretreatment enhances the therapeutic efficacy of platelet-membrane-camouflaged antitumor nanoparticles. J Nanobiotechnology 18:101 (2020). PubMed: 32690018
  • Eser Ocak P  et al. Overexpression of Mfsd2a attenuates blood brain barrier dysfunction via Cav-1/Keap-1/Nrf-2/HO-1 pathway in a rat model of surgical brain injury. Exp Neurol 326:113203 (2020). PubMed: 31954682
  • Han N  et al. MicroRNA-203 inhibits epithelial-mesenchymal transition, migration, and invasion of renal cell carcinoma cells via the inactivation of the PI3K/AKT signaling pathway by inhibiting CAV1. Cell Adh Migr 14:227-241 (2020). PubMed: 32990143
  • Peng W  et al. LINC81507 act as a competing endogenous RNA of miR-199b-5p to facilitate NSCLC proliferation and metastasis via regulating the CAV1/STAT3 pathway. Cell Death Dis 10:533 (2019). PubMed: 31296840
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caveolin-1 antibody [E249] - Caveolae Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA-Buffer pH 9,0
Permeabilization
Yes - Wash Buffer from Dako with Tween
Specification
Kidney
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Rudolf Jung

Verified customer

Submitted Jun 17 2016

Western blot abreview for Anti-Caveolin 1 antibody [E249]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC)
Loading amount
100000 cells
Specification
HUVEC
Treatment
various doses of VEGF for 24h
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 05 2008

Question

I have purchased several anti-bodies from abcam:
ab83015
ab79397
ab81127
ab48139
ab32577
ab98975.
I have used the products WB but would now like to use them for IHC. The website says that they have not been tested in this application. Do you know how likely these Abs will be in succeeding if I attempt to do this
Any help would be much appreaciated.

Read More

Abcam community

Verified customer

Asked on Feb 17 2012

Answer

Thank you for contacting us.

Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen sections, but they are very likely to react in such conditions.

Ab48139 has recently been validated to use in IHC-P in Canine, Feline, Mouse, Rat and Human.

As for ab81127, ab83015 and ab98975, unfortunately, we have not characterised them by IHC, it doesn’t mean they won’t work in this application, but we don’t have data to support it.

At Abcam we have an antibody evaluation system, called Abreview, by which customers interested in testing an antibody in new species or applications (not yet tested) can benefit from a free primary antibody in their next purchase if they share the results with us, publishing them in our website.

To participate in this system, customers should first let us know the antibody they want to test in the new species/ applications. We then issue the discount code valid for one free primary antibody. This code must be issued before purchasing the antibody. The customer then purchases the antibody normally, and tests it in the new species / applications.

Once we receive the results in our Website as an Abreview, the discount code becomes active, and it can be redeemed for any primary antibody ordered within the next 4 months.

As mentioned, the discount code might be issued before the purchase of the antibody, but, as an exceptional case, I can still send a discount code if you would like to test any of these antibodies (ab81127, ab83015 and ab98975) with IHC and share your results with us.

To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: http://it.adminsite.com/admin/crm2/www.abcam.com/collaborationdiscount.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Feb 17 2012

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