Overview

  • Product name

    Anti-Caveolin-1 antibody - N-terminal
    See all Caveolin-1 primary antibodies
  • Description

    Goat polyclonal to Caveolin-1 - N-terminal
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Dog, Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment within Human Caveolin-1 aa 1-100 (N terminal). The exact sequence is proprietary. (NP_001744.2).
    Database link: Q03135

  • Positive control

    • HaCat, MDCK and 3T3 cell lysates; Human heart tissue; Hepa1-6 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab211503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 20 kDa).
ICC/IF 1/25 - 1/250.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • Database links

  • Alternative names

    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Images

  • Anti-Caveolin-1 antibody - N-terminal (ab211503) at 1/1000 dilution + HaCat cell lysate at 100 µg

    Secondary
    Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution

    Predicted band size: 20 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue labeling Caveolin-1 with ab211503 at 5 µg/ml.

  • Immunofluorescent analysis of 4% PFA-fixed Hepa1-6 cells labeling Caveolin-1 with ab211503 at 1/50 dilution.

References

ab211503 has not yet been referenced specifically in any publications.

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