Key features and details
- Goat polyclonal to Caveolin-1 - N-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Dog, Human
- Isotype: IgG
Product nameAnti-Caveolin-1 antibody - N-terminal
See all Caveolin-1 primary antibodies
DescriptionGoat polyclonal to Caveolin-1 - N-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Dog, Human
Predicted to work with: Monkey
Recombinant fragment within Human Caveolin-1 aa 1-100 (N terminal). The exact sequence is proprietary. (NP_001744.2).
Database link: Q03135
- HaCat, MDCK and 3T3 cell lysates; Human heart tissue; Hepa1-6 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 20% Glycerol, 79% PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab211503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 20 kDa).|
|ICC/IF||1/25 - 1/250.|
FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Tissue specificityExpressed in muscle and lung, less so in liver, brain and kidney.
Involvement in diseaseDefects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Sequence similaritiesBelongs to the caveolin family.
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
Cellular localizationGolgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
- Information by UniProt
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
Anti-Caveolin-1 antibody - N-terminal (ab211503) at 1/1000 dilution + HaCat cell lysate at 100 µg
Rabbit polyclonal to goat IgG (HRP) at 1/10000 dilution
Predicted band size: 20 kDa
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human heart tissue labeling Caveolin-1 with ab211503 at 5 µg/ml.
Immunofluorescent analysis of 4% PFA-fixed Hepa1-6 cells labeling Caveolin-1 with ab211503 at 1/50 dilution.
ab211503 has not yet been referenced specifically in any publications.