Recombinant
RabMAb

Recombinant Anti-Caveolin-1 antibody [SP43] - BSA and Azide free (ab236437)

Overview

  • Product name

    Anti-Caveolin-1 antibody [SP43] - BSA and Azide free
    See all Caveolin-1 primary antibodies
  • Description

    Rabbit monoclonal [SP43] to Caveolin-1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide within Human Caveolin-1 (C terminal). The exact sequence is proprietary.
    Database link: Q03135

  • Positive control

    • IHC-P: Human lung carcinoma, placenta, liver hepatocellular carcinoma, lung, skeletal muscle, liver, cerebellum, cerebral cortex, colon and HK lymphoma tissues
  • General notes

    Ab236437 is the carrier-free version of ab227636. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab236437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • Database links

  • Alternative names

    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling Caveolin-1 with ab227636 at 1/50 dilution (4.70 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human lung, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227636 for 30 mins at room temperature. This image was generated using ab227636, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Caveolin-1 with ab227636 at 1/50 dilution (4.70 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells in the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227636 for 30 mins at room temperature. This image was generated using ab227636, the same clone, but with a different buffer formulation.

  • Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

  • Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

  • Formalin-fixed, paraffin-embedded human lung tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

  • Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

  • Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

  • Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

  • Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227636).

References

ab236437 has not yet been referenced specifically in any publications.

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