Overview

  • Product name

    Anti-Caveolin-1 antibody [SP43] - C-terminal
    See all Caveolin-1 primary antibodies
  • Description

    Rabbit monoclonal [SP43] to Caveolin-1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide within Human Caveolin-1 aa 150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q03135

  • Positive control

    • IHC-P: Human lung carcinoma, placenta, liver hepatocellular carcinoma, lung, skeletal muscle, liver, cerebellum, cerebral cortex, colon and HK lymphoma tissues

Properties

Applications

Our Abpromise guarantee covers the use of ab227636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 30 minutes at room temperature.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Information by UniProt
  • Database links

  • Alternative names

    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung tissue sections labeling Caveolin-1 with ab227636 at 1/50 dilution (4.70 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human lung, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227636 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling Caveolin-1 with ab227636 at 1/50 dilution (4.70 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the stromal cells in the human lung carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227636 for 30 mins at room temperature.

  • Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human placenta tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver hepatocellular carcinoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human lung tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human colon tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

  • Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Caveolin-1 using ab227636 at 1/50 dilution in immunohistochemical analysis.

References

ab227636 has not yet been referenced specifically in any publications.

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