Product nameAnti-Caveolin-1 (phospho Y14) antibody
See all Caveolin-1 primary antibodies
DescriptionRabbit polyclonal to Caveolin-1 (phospho Y14)
SpecificityThis antibody is specific for Caveolin-1 only when phosphorylated at tyrosine 14.
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Rat, Cow, Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human Caveolin-1.
Database link: Q03135
- NIH 3T3 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Our Abpromise guarantee covers the use of ab38468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).|
FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
Tissue specificityExpressed in muscle and lung, less so in liver, brain and kidney.
Involvement in diseaseDefects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
Sequence similaritiesBelongs to the caveolin family.
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
Cellular localizationGolgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
- Information by UniProt
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
Lanes 1-2 : Caveolin-1 Antibody
Lanes 3-4 : Anti-Caveolin-1 (phospho Y14) antibody (ab38468) at 1/500 dilution
Lane 1 : NIH 3T3 cell extract
Lane 2 : NIH 3T3 cell extract. Pre-incubated with synthesized peptide
Lane 3 : NIH 3T3 cell extract.
Lane 4 : NIH 3T3 cell extract. Treated with H2O2.
Predicted band size: 20 kDa
Observed band size: 20 kDa