Overview

  • Product name

  • Description

    Rabbit polyclonal to Caveolin-2
  • Host species

    Rabbit
  • Specificity

    Detects caveolin-2 from rat tissues. This antibody does not detect caveolin-1 or -3.
  • Tested applications

    Suitable for: WB, IP, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Sheep, Rabbit, Cow, Cat, Pig, Chimpanzee, Gorilla, African green monkey, African bush elephant
  • Immunogen

    Synthetic peptide corresponding to Rat Caveolin-2 aa 1-19.
    Sequence:

    MGLETEKADVQLFMADDAY


    Database link: Q2IBC5
    (Peptide available as ab4929)

Properties

Applications

Our Abpromise guarantee covers the use of ab2911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Can be blocked with Caveolin-2 peptide (ab4929).

Can be blocked with Caveolin-2 peptide.

IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 20130741
ICC/IF 1/200.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
  • Tissue specificity

    Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
  • Cellular localization

    Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAV antibody
    • CAV2 antibody
    • CAV2_HUMAN antibody
    • Caveolae protein 20 kD antibody
    • Caveolin 2 antibody
    • Caveolin 2 isoform a and b antibody
    • Caveolin-2 antibody
    • MGC12294 antibody
    • OTTHUMP00000025032 antibody
    • OTTHUMP00000195982 antibody
    see all

Images

  •  Western blot of caveolin-2 on rat skeletal muscle protein extract using ab2911.

  • IHC image of ab2911 staining in human normal cervix formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2911, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Mortensen OV  et al. MAP Kinase Phosphatase 3 (MKP3) Preserves Norepinephrine Transporter Activity by Modulating ERK1/2 Kinase-Mediated Gene Expression. Front Cell Neurosci 11:253 (2017). Read more (PubMed: 28878626) »
  • Hoeke L  et al. Intestinal Salmonella typhimurium infection leads to miR-29a induced caveolin 2 regulation. PLoS One 8:e67300 (2013). WB . Read more (PubMed: 23826261) »
See all 8 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

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1-4 of 4 Abreviews

Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 22 2018

Application
Immunocytochemistry
Sample
Rat Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 16 2018

Application
Western blot
Sample
Pig Tissue lysate - whole (Colon)
Loading amount
20 µg
Specification
Colon
Gel Running Conditions
Reduced Denaturing (Trizingel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%

Abcam user community

Verified customer

Submitted Sep 29 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293, Kidney)
Loading amount
100 µg
Specification
HEK 293, Kidney
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C

Dr. Olga Roche

Verified customer

Submitted Dec 19 2007

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