Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2220] to Caveolin-2 - Caveolae Marker
- Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-Caveolin-2 antibody [EPR2220] - Caveolae Marker
See all Caveolin-2 primary antibodies
DescriptionRabbit monoclonal [EPR2220] to Caveolin-2 - Caveolae Marker
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Caveolin-2. The exact sequence is proprietary.
Database link: P51636
- WB: HeLa cell lysate. IHC-P: Human uterus tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab79397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/10000. Predicted molecular weight: 18 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
Tissue specificityExpressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
Sequence similaritiesBelongs to the caveolin family.
modificationsPhosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
Cellular localizationNucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
- Information by UniProt
- CAV antibody
- CAV2 antibody
- CAV2_HUMAN antibody
Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397) at 1/5000 dilution + HeLa cell lysate at 10 µg
Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
ab79397 at 1/100 dilution staining Caveolin-2 in human uterus by Immunohistochemistry using paraffin-embedded tissue. Note staining of endothelial cells and smooth muscles.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab79397 at 1/100 dilution staining Caveolin-2 in HeLa cells by Immunofluorescence.
Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caveolin-2 (red) with ab79397 at a 1/2000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
ab79397 has been referenced in 1 publication.
- Hashani M et al. Widespread expression of perilipin 5 in normal human tissues and in diseases is restricted to distinct lipid droplet subpopulations. Cell Tissue Res N/A:N/A (2018). Human . PubMed: 29752569