Recombinant Anti-Caveolin-2 antibody [EPR5471] (ab133484)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Caveolin-2 antibody [EPR5471]
See all Caveolin-2 primary antibodies -
Description
Rabbit monoclonal [EPR5471] to Caveolin-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Caveolin-2 (C terminal). The exact sequence is proprietary.
Database link: P51636 -
Positive control
- HUVEC, HeLa, 3T3-L1, and U87-MG cell lysates.
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General notes
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR5471 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 18 kDa. | |
| ICC/IF | 1/50. |
Target
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Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression. -
Tissue specificity
Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts. -
Sequence similarities
Belongs to the caveolin family. -
Post-translational
modificationsPhosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin. -
Cellular localization
Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments. - Information by UniProt
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Database links
- Entrez Gene: 858 Human
- Entrez Gene: 12390 Mouse
- Entrez Gene: 100362824 Rat
- Entrez Gene: 363425 Rat
- Omim: 601048 Human
- SwissProt: P51636 Human
- SwissProt: Q9WVC3 Mouse
- SwissProt: Q2IBC5 Rat
see all -
Alternative names
- CAV antibody
- CAV2 antibody
- CAV2_HUMAN antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)](https://www.abcam.com/ps/products/133/ab133484/Images/ab133484-236065-anti-caveolin-2-antibody-epr5471-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)ab133484 stained MCF cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133484 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)](https://www.abcam.com/ps/products/133/ab133484/Images/ab133484-147134-anti-caveolin-2-antibody-epr5471-western-blot.jpg)
Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)All lanes : Anti-Caveolin-2 antibody [EPR5471] (ab133484) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 3T3-L1 cell lysate
Lane 4 : U87-MG cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
Datasheets and documents
References
ab133484 has not yet been referenced specifically in any publications.
