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  1. Link

    caveolin-2-antibody-epr5471-ab133484.pdf

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Tags & Cell Markers Subcellular Markers Organelles Caveolae and Clathrin
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RecombinantRabMAb

Recombinant Anti-Caveolin-2 antibody [EPR5471] (ab133484)

  • Datasheet
Reviews (1) Submit a question References (4)

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Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)
  • Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)
  • Anti-Caveolin-2 antibody [EPR5471] (ab133484)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5471] to Caveolin-2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Human Caveolin-2 protein (ab114515)
Primary
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Anti-C14orf133 antibody (ab125084)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Caveolin-2 antibody [EPR5471]
    See all Caveolin-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR5471] to Caveolin-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide within Human Caveolin-2 (C terminal). The exact sequence is proprietary.
    Database link: P51636

  • Positive control

    • HUVEC, HeLa, 3T3-L1, and U87-MG cell lysates.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5471
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cardiovascular
    • Atherosclerosis
    • Lipid transport
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis

Associated products

  • Alternative Versions

    • Anti-Caveolin-2 antibody [EPR5471] - BSA and Azide free (ab236058)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • HeLa membrane extract lysate (ab29547)
  • Recombinant Protein

    • Recombinant Human Caveolin-2 protein (ab114515)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133484 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
ICC/IF
1/50.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
ICC/IF
1/50.
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
  • Tissue specificity

    Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
  • Cellular localization

    Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Target information above from: UniProt accession P51636 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 858 Human
    • Entrez Gene: 12390 Mouse
    • Entrez Gene: 100362824 Rat
    • Entrez Gene: 363425 Rat
    • Omim: 601048 Human
    • SwissProt: P51636 Human
    • SwissProt: Q9WVC3 Mouse
    • SwissProt: Q2IBC5 Rat
    • Unigene: 212332 Human
    • Unigene: 603096 Human
    • Unigene: 396075 Mouse
    • Unigene: 81070 Rat
    see all
  • Alternative names

    • CAV antibody
    • CAV2 antibody
    • CAV2_HUMAN antibody
    • Caveolae protein 20 kD antibody
    • Caveolin 2 antibody
    • Caveolin 2 isoform a and b antibody
    • Caveolin-2 antibody
    • MGC12294 antibody
    • OTTHUMP00000025032 antibody
    • OTTHUMP00000195982 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)
    Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR5471] (ab133484)

    ab133484 stained MCF cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab133484 at 1/100 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)
    Western blot - Anti-Caveolin-2 antibody [EPR5471] (ab133484)
    All lanes : Anti-Caveolin-2 antibody [EPR5471] (ab133484) at 1/1000 dilution

    Lane 1 : HUVEC cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : 3T3-L1 cell lysate
    Lane 4 : U87-MG cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 18 kDa

  • Anti-Caveolin-2 antibody [EPR5471] (ab133484)
    Anti-Caveolin-2 antibody [EPR5471] (ab133484)

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (4)

Publishing research using ab133484? Please let us know so that we can cite the reference in this datasheet.

ab133484 has been referenced in 4 publications.

  • Li J  et al. CircRNA TADA2A relieves idiopathic pulmonary fibrosis by inhibiting proliferation and activation of fibroblasts. Cell Death Dis 11:553 (2020). PubMed: 32694556
  • Schmidt F  et al. Flotillin-Dependent Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal Infections. Cell Rep 32:108017 (2020). PubMed: 32814035
  • Wang Z  et al. NEAT1 regulates neuroglial cell mediating Aß clearance via the epigenetic regulation of endocytosis-related genes expression. Cell Mol Life Sci N/A:N/A (2019). PubMed: 31006037
  • Wang C  et al. Endocytosis of flavivirus NS1 is required for NS1-mediated endothelial hyperpermeability and is abolished by a single N-glycosylation site mutation. PLoS Pathog 15:e1007938 (2019). PubMed: 31356638

Customer reviews and Q&As

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Western blot abreview for Anti-Caveolin-2 antibody [EPR5471]

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (Duodenum Cytosolic Lipid Droplets)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Duodenum Cytosolic Lipid Droplets
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Jun 01 2017

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