Western blot abreview for Anti-Caveolin 2 (phospho Y19) antibody

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Fibroblasts)
Loading amount
50 µg
Specification
Fibroblasts
Treatment
100 nM insulin for 10, 30, 60, 120, and 180 min
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 5% BSA

Secondary antibody

Name
Non-Abcam antibody was used: Promega anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/15000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 20 kDa Non-specific: 30~ kDa
Negative control
IgG isotype control Ab

Additional data

PubMed ID
19427337: View

Dr. Y Pak

Verified customer

Submitted Aug 07 2009

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