Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin 2 (phospho Y19) antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Fibroblasts)
Specification
Fibroblasts
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C

Other product details

Dilution
1/250
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA/PBS

Secondary antibody

Name
Non-Abcam antibody was used: Sigma anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: TRITC
Dilution
1/100

Additional data

PubMed ID
19778377: View
Additional Notes
(a) Control cells untreated cells
(b) 100nM Insulin for 10 min
(c) 10uM U0126 for 2 hr and 100nM Insulin for 10 min
(d) 100 nM Wortmannin for 1hr and 100nM Insulin for 10 min

Dr. Y Pak

Verified customer

Submitted Mar 01 2010

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