Western blot abreview for Anti-Caveolin 2 (phospho Y19) antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat)
Loading amount
1e+006 cells
Specification
Jurkat
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: goat anti rabbit hrp
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ecl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 21 kDa Non-specific: 50, 80, 120, smear kDa
Negative control
bsa

Additional data

Additional Notes
blocking with bsa gives higher background than with milk

Abcam user community

Verified customer

Submitted Jul 28 2006

Sign up