Key features and details
- Rabbit monoclonal [EP2221Y] to Caveolin-2 (phospho Y27)
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Caveolin-2 (phospho Y27) antibody [EP2221Y]
See all Caveolin-2 primary antibodies
DescriptionRabbit monoclonal [EP2221Y] to Caveolin-2 (phospho Y27)
SpecificityThis antibody detects Caveolin-2 phosphorylated at tyrosine 27.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Synthetic peptide within Human Caveolin-2. The exact sequence is proprietary.
Database link: P51636
- Hela cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab76405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Predicted molecular weight: 18 kDa.|
FunctionMay act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
Tissue specificityExpressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
Sequence similaritiesBelongs to the caveolin family.
modificationsPhosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
Cellular localizationNucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
- Information by UniProt
- CAV antibody
- CAV2 antibody
- CAV2_HUMAN antibody
All lanes : Anti-Caveolin-2 (phospho Y27) antibody [EP2221Y] (ab76405) at 1/2000 dilution
Lane 1 : HeLa cell lysates untreated.
Lane 2 : HeLa cell lysates treated with pervanadate.
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 18 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
ab76405 has not yet been referenced specifically in any publications.